Estimation of the degree of self-incompatibility reaction during flower bud development and production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars

Akira Wakana, Binh Xuan Ngo, Isao Fukudome, Kohei Kajiwara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bud pollination in seven self-incompatible Citrus cultivars was carried out to produce self-fertilized seedlings with genotypes homozygous for the self-incompatibility gene. Flower buds of the seven self-incompatible Citrus cultivars, 'Banpeiyu' (C. grandis), 'Hirado Buntan' (C. grandis), 'Hassaku' (C. hassaku), 'Shishiyuzu' (C. pseudogulgul), 'Clementine' (C. clementina), 'Tosa Buntan' (C. otachibana) and 'Hyuganatsu' (C. tamurana), were emasculated at various stages of flower bud development, and self-pollinated with their fresh pollen from flower buds collected just before anthesis. Seeded fruits were obtained from flower buds of 6-8 mm length for 'Clementine', 7-11 mm for 'Hyuganatsu', 11-15 mm for 'Shishiyuzu', 6-14 mm for 'Hassaku', 12-19 mm for 'Hirado Buntan' and 12-22 mm for 'Banpeiyu'. The optimum length of flower buds for self-fertilized seed production was 6-8 mm for 'Clementine', 8-12 mm for 'Hassaku', 9-13 mm for 'Hyuganatsu', 11-14 mm for 'Shishiyuzu', 12-17 mm for 'Hirado' and 14-19 mm for 'Banpeiyu'. Flower buds producing the largest number of perfect seeds in each self-pollination were about half the length of those just before anthesis. At or just after this stage of flower bud development, stigmatic exudate production occurred. The largest number of perfect seeds in self-pollinated fruits was almost the same as the average number of perfect seeds in open- or cross-pollinated fruits in six cultivars except for 'Shishiyuzu' in which self-pollinated fruits contained a few seeds. These results suggested that half-sized flower buds in their development were the most optimum stage for the production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars, and that self-incompatibility reaction increased after this stage. It was also suggested that in 'Shishiyuzu' the self-incompatibility reaction is different from that in the other six Citrus cultivars. No difference was found in size between self-fertilized and cross-fertilized seeds. The self-fertilized seeds germinated and grew normally without any inbreeding depression.

Original languageEnglish
Pages (from-to)307-320
Number of pages14
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume49
Issue number2
Publication statusPublished - Oct 1 2004

Fingerprint

Pollination
Citrus
self-pollination
Seeds
buds
cultivars
seeds
Fruit
clementines
Citrus maxima
fruits
seed productivity
flowering
Exudates and Transudates
Pollen
Seedlings
inbreeding depression
homozygosity
Genotype
pollination

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Estimation of the degree of self-incompatibility reaction during flower bud development and production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars. / Wakana, Akira; Ngo, Binh Xuan; Fukudome, Isao; Kajiwara, Kohei.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 49, No. 2, 01.10.2004, p. 307-320.

Research output: Contribution to journalArticle

@article{0278de40a4c24f18a00effd5b1ee98fe,
title = "Estimation of the degree of self-incompatibility reaction during flower bud development and production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars",
abstract = "Bud pollination in seven self-incompatible Citrus cultivars was carried out to produce self-fertilized seedlings with genotypes homozygous for the self-incompatibility gene. Flower buds of the seven self-incompatible Citrus cultivars, 'Banpeiyu' (C. grandis), 'Hirado Buntan' (C. grandis), 'Hassaku' (C. hassaku), 'Shishiyuzu' (C. pseudogulgul), 'Clementine' (C. clementina), 'Tosa Buntan' (C. otachibana) and 'Hyuganatsu' (C. tamurana), were emasculated at various stages of flower bud development, and self-pollinated with their fresh pollen from flower buds collected just before anthesis. Seeded fruits were obtained from flower buds of 6-8 mm length for 'Clementine', 7-11 mm for 'Hyuganatsu', 11-15 mm for 'Shishiyuzu', 6-14 mm for 'Hassaku', 12-19 mm for 'Hirado Buntan' and 12-22 mm for 'Banpeiyu'. The optimum length of flower buds for self-fertilized seed production was 6-8 mm for 'Clementine', 8-12 mm for 'Hassaku', 9-13 mm for 'Hyuganatsu', 11-14 mm for 'Shishiyuzu', 12-17 mm for 'Hirado' and 14-19 mm for 'Banpeiyu'. Flower buds producing the largest number of perfect seeds in each self-pollination were about half the length of those just before anthesis. At or just after this stage of flower bud development, stigmatic exudate production occurred. The largest number of perfect seeds in self-pollinated fruits was almost the same as the average number of perfect seeds in open- or cross-pollinated fruits in six cultivars except for 'Shishiyuzu' in which self-pollinated fruits contained a few seeds. These results suggested that half-sized flower buds in their development were the most optimum stage for the production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars, and that self-incompatibility reaction increased after this stage. It was also suggested that in 'Shishiyuzu' the self-incompatibility reaction is different from that in the other six Citrus cultivars. No difference was found in size between self-fertilized and cross-fertilized seeds. The self-fertilized seeds germinated and grew normally without any inbreeding depression.",
author = "Akira Wakana and Ngo, {Binh Xuan} and Isao Fukudome and Kohei Kajiwara",
year = "2004",
month = "10",
day = "1",
language = "English",
volume = "49",
pages = "307--320",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "2",

}

TY - JOUR

T1 - Estimation of the degree of self-incompatibility reaction during flower bud development and production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars

AU - Wakana, Akira

AU - Ngo, Binh Xuan

AU - Fukudome, Isao

AU - Kajiwara, Kohei

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Bud pollination in seven self-incompatible Citrus cultivars was carried out to produce self-fertilized seedlings with genotypes homozygous for the self-incompatibility gene. Flower buds of the seven self-incompatible Citrus cultivars, 'Banpeiyu' (C. grandis), 'Hirado Buntan' (C. grandis), 'Hassaku' (C. hassaku), 'Shishiyuzu' (C. pseudogulgul), 'Clementine' (C. clementina), 'Tosa Buntan' (C. otachibana) and 'Hyuganatsu' (C. tamurana), were emasculated at various stages of flower bud development, and self-pollinated with their fresh pollen from flower buds collected just before anthesis. Seeded fruits were obtained from flower buds of 6-8 mm length for 'Clementine', 7-11 mm for 'Hyuganatsu', 11-15 mm for 'Shishiyuzu', 6-14 mm for 'Hassaku', 12-19 mm for 'Hirado Buntan' and 12-22 mm for 'Banpeiyu'. The optimum length of flower buds for self-fertilized seed production was 6-8 mm for 'Clementine', 8-12 mm for 'Hassaku', 9-13 mm for 'Hyuganatsu', 11-14 mm for 'Shishiyuzu', 12-17 mm for 'Hirado' and 14-19 mm for 'Banpeiyu'. Flower buds producing the largest number of perfect seeds in each self-pollination were about half the length of those just before anthesis. At or just after this stage of flower bud development, stigmatic exudate production occurred. The largest number of perfect seeds in self-pollinated fruits was almost the same as the average number of perfect seeds in open- or cross-pollinated fruits in six cultivars except for 'Shishiyuzu' in which self-pollinated fruits contained a few seeds. These results suggested that half-sized flower buds in their development were the most optimum stage for the production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars, and that self-incompatibility reaction increased after this stage. It was also suggested that in 'Shishiyuzu' the self-incompatibility reaction is different from that in the other six Citrus cultivars. No difference was found in size between self-fertilized and cross-fertilized seeds. The self-fertilized seeds germinated and grew normally without any inbreeding depression.

AB - Bud pollination in seven self-incompatible Citrus cultivars was carried out to produce self-fertilized seedlings with genotypes homozygous for the self-incompatibility gene. Flower buds of the seven self-incompatible Citrus cultivars, 'Banpeiyu' (C. grandis), 'Hirado Buntan' (C. grandis), 'Hassaku' (C. hassaku), 'Shishiyuzu' (C. pseudogulgul), 'Clementine' (C. clementina), 'Tosa Buntan' (C. otachibana) and 'Hyuganatsu' (C. tamurana), were emasculated at various stages of flower bud development, and self-pollinated with their fresh pollen from flower buds collected just before anthesis. Seeded fruits were obtained from flower buds of 6-8 mm length for 'Clementine', 7-11 mm for 'Hyuganatsu', 11-15 mm for 'Shishiyuzu', 6-14 mm for 'Hassaku', 12-19 mm for 'Hirado Buntan' and 12-22 mm for 'Banpeiyu'. The optimum length of flower buds for self-fertilized seed production was 6-8 mm for 'Clementine', 8-12 mm for 'Hassaku', 9-13 mm for 'Hyuganatsu', 11-14 mm for 'Shishiyuzu', 12-17 mm for 'Hirado' and 14-19 mm for 'Banpeiyu'. Flower buds producing the largest number of perfect seeds in each self-pollination were about half the length of those just before anthesis. At or just after this stage of flower bud development, stigmatic exudate production occurred. The largest number of perfect seeds in self-pollinated fruits was almost the same as the average number of perfect seeds in open- or cross-pollinated fruits in six cultivars except for 'Shishiyuzu' in which self-pollinated fruits contained a few seeds. These results suggested that half-sized flower buds in their development were the most optimum stage for the production of self-fertilized seeds by bud pollination in self-incompatible Citrus cultivars, and that self-incompatibility reaction increased after this stage. It was also suggested that in 'Shishiyuzu' the self-incompatibility reaction is different from that in the other six Citrus cultivars. No difference was found in size between self-fertilized and cross-fertilized seeds. The self-fertilized seeds germinated and grew normally without any inbreeding depression.

UR - http://www.scopus.com/inward/record.url?scp=10044253039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044253039&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:10044253039

VL - 49

SP - 307

EP - 320

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 2

ER -