Estimation of the performance of a PUF/spheroid packed-bed type artificial liver by using an extracorporeal circulation with hepatic failure rats

T. Matsushita, Hiroyuki Ijima, S. Wada, K. Funatsu

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Abstract

Multicapillary (MC) polyurethane foam (PUF)/spheroid packed-bed type artificial liver was applied to cure D-galactosamine induced hepatic failure rats (PT value; 27-43 [s] in failure, ;19-20 [s] in normal) with an extracorporeal circulation for 3 hours, which connected with the carotid artery and jugular vein (n=5). In 4 cases of the experiments, the increasing of the blood ammonia concentrations were suppressed under hepatic coma level (196 N-μg/dl) and the concentrations decreased at normal level within 40 hours. The increased PT, GPT, GOT, total bilirubin, and total bile acid concentrations in the blood also decreased at normal level within 4 days, and these rats were recovered from hepatic failure. On the contrary, when MC-PUF packed-bed without hepatocytes were applied to the hepatic failure rats (n=3, control experiments), the blood ammonia concentrations rapidly increased more than hepatic coma level (196 N-μg/dl) in all cases and these rats were died. From these results, it was suggested that hepatocytes/spheroid in the artificial liver could detoxify the blood ammonia, and had some important roles for recovery from D-gal induced hepatic failure.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
JournalJapanese Journal of Artificial Organs
Volume24
Issue number3
Publication statusPublished - 1995

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Artificial Liver
Extracorporeal Circulation
Liver Failure
Ammonia
Hepatic Encephalopathy
Hepatocytes
Galactosamine
Jugular Veins
Bile Acids and Salts
Carotid Arteries
Bilirubin
polyurethane foam

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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title = "Estimation of the performance of a PUF/spheroid packed-bed type artificial liver by using an extracorporeal circulation with hepatic failure rats",
abstract = "Multicapillary (MC) polyurethane foam (PUF)/spheroid packed-bed type artificial liver was applied to cure D-galactosamine induced hepatic failure rats (PT value; 27-43 [s] in failure, ;19-20 [s] in normal) with an extracorporeal circulation for 3 hours, which connected with the carotid artery and jugular vein (n=5). In 4 cases of the experiments, the increasing of the blood ammonia concentrations were suppressed under hepatic coma level (196 N-μg/dl) and the concentrations decreased at normal level within 40 hours. The increased PT, GPT, GOT, total bilirubin, and total bile acid concentrations in the blood also decreased at normal level within 4 days, and these rats were recovered from hepatic failure. On the contrary, when MC-PUF packed-bed without hepatocytes were applied to the hepatic failure rats (n=3, control experiments), the blood ammonia concentrations rapidly increased more than hepatic coma level (196 N-μg/dl) in all cases and these rats were died. From these results, it was suggested that hepatocytes/spheroid in the artificial liver could detoxify the blood ammonia, and had some important roles for recovery from D-gal induced hepatic failure.",
author = "T. Matsushita and Hiroyuki Ijima and S. Wada and K. Funatsu",
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AU - Matsushita, T.

AU - Ijima, Hiroyuki

AU - Wada, S.

AU - Funatsu, K.

PY - 1995

Y1 - 1995

N2 - Multicapillary (MC) polyurethane foam (PUF)/spheroid packed-bed type artificial liver was applied to cure D-galactosamine induced hepatic failure rats (PT value; 27-43 [s] in failure, ;19-20 [s] in normal) with an extracorporeal circulation for 3 hours, which connected with the carotid artery and jugular vein (n=5). In 4 cases of the experiments, the increasing of the blood ammonia concentrations were suppressed under hepatic coma level (196 N-μg/dl) and the concentrations decreased at normal level within 40 hours. The increased PT, GPT, GOT, total bilirubin, and total bile acid concentrations in the blood also decreased at normal level within 4 days, and these rats were recovered from hepatic failure. On the contrary, when MC-PUF packed-bed without hepatocytes were applied to the hepatic failure rats (n=3, control experiments), the blood ammonia concentrations rapidly increased more than hepatic coma level (196 N-μg/dl) in all cases and these rats were died. From these results, it was suggested that hepatocytes/spheroid in the artificial liver could detoxify the blood ammonia, and had some important roles for recovery from D-gal induced hepatic failure.

AB - Multicapillary (MC) polyurethane foam (PUF)/spheroid packed-bed type artificial liver was applied to cure D-galactosamine induced hepatic failure rats (PT value; 27-43 [s] in failure, ;19-20 [s] in normal) with an extracorporeal circulation for 3 hours, which connected with the carotid artery and jugular vein (n=5). In 4 cases of the experiments, the increasing of the blood ammonia concentrations were suppressed under hepatic coma level (196 N-μg/dl) and the concentrations decreased at normal level within 40 hours. The increased PT, GPT, GOT, total bilirubin, and total bile acid concentrations in the blood also decreased at normal level within 4 days, and these rats were recovered from hepatic failure. On the contrary, when MC-PUF packed-bed without hepatocytes were applied to the hepatic failure rats (n=3, control experiments), the blood ammonia concentrations rapidly increased more than hepatic coma level (196 N-μg/dl) in all cases and these rats were died. From these results, it was suggested that hepatocytes/spheroid in the artificial liver could detoxify the blood ammonia, and had some important roles for recovery from D-gal induced hepatic failure.

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