Estrogen differentially regulates expression of Per1 and Per2 genes between central and peripheral clocks and between reproductive and nonreproductive tissues in female rats

Takahiro J. Nakamura, Takahiro Moriya, Shin Inoue, Takao Shimazoe, Shigenori Watanabe, Shizufumi Ebihara, Kazuyuki Shinohara

Research output: Contribution to journalArticle

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Abstract

Although it has long been established that estrogen alters circadian rhythms in behavior, physiology, and reproductive functions in mammals, the molecular mechanism for these effects remains unknown. To explore the possibility that estrogen affects circadian rhythms by changing the expression of clock-related genes, we investigated the effects of chronic treatment with 17β-estradiol (E2) on the expression of Per1 and Per2 genes in the brain (suprachiasmatic nucleus and cerebral cortex) and periphery (liver, kidney, and uterus) of ovariectomized rats by means of in situ hybridization and northern blotting. In the brain, E2 treatment advanced the peak of Per2 mRNA expression in the SCN; however, it failed to affect the rhythm of Per2 mRNA expression in the CX and Per1 mRNA expression in both the SCN and the CX. In nonreproductive peripheral tissues (liver and kidney), E2 delayed the phase and increased the amplitude of Per1 mRNA expression. In the reproductive tissues (uterus), biphasic rhythms in Per1 and Per2 mRNA were observed after E2 treatment. These findings suggest that the effects of estrogen are different between central and peripheral clock in the brain, and between reproductive and nonreproductive tissues in the periphery.

Original languageEnglish
Pages (from-to)622-630
Number of pages9
JournalJournal of Neuroscience Research
Volume82
Issue number5
DOIs
Publication statusPublished - Dec 1 2005

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Estrogens
Messenger RNA
Genes
Circadian Rhythm
Uterus
Brain
Kidney
Reproductive Behavior
Suprachiasmatic Nucleus
Liver
Northern Blotting
Cerebral Cortex
In Situ Hybridization
Mammals
Estradiol

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Estrogen differentially regulates expression of Per1 and Per2 genes between central and peripheral clocks and between reproductive and nonreproductive tissues in female rats. / Nakamura, Takahiro J.; Moriya, Takahiro; Inoue, Shin; Shimazoe, Takao; Watanabe, Shigenori; Ebihara, Shizufumi; Shinohara, Kazuyuki.

In: Journal of Neuroscience Research, Vol. 82, No. 5, 01.12.2005, p. 622-630.

Research output: Contribution to journalArticle

Nakamura, Takahiro J. ; Moriya, Takahiro ; Inoue, Shin ; Shimazoe, Takao ; Watanabe, Shigenori ; Ebihara, Shizufumi ; Shinohara, Kazuyuki. / Estrogen differentially regulates expression of Per1 and Per2 genes between central and peripheral clocks and between reproductive and nonreproductive tissues in female rats. In: Journal of Neuroscience Research. 2005 ; Vol. 82, No. 5. pp. 622-630.
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