Eukaryotic peptide chain release factor 1 participates in translation termination of specific cysteine-poor prolamines in rice endosperm

Ammar Elakhdar, Tomokazu Ushijima, Masako Fukuda, Noriko Yamashiro, Yasushi Kawagoe, Toshihiro Kumamaru

Research output: Contribution to journalArticle

Abstract

Prolamines are alcohol-soluble proteins classified as either cysteine-poor (CysP) or cysteine-rich (CysR) based on whether they can be alcohol-extracted without or with reducing agents, respectively. In rice esp1 mutants, various CysP prolamines exhibit both reduced and normal amounts of isoelectric focusing bands, indicating that the mutation affects only certain prolamine classes. To examine the genetic regulation of CysP prolamine synthesis and accumulation, we constructed a high-resolution genetic linkage map of ESP1. The ESP1 gene was mapped to within a 20 kb region on rice chromosome 7. Sequencing analysis of annotated genes in this region revealed a single-nucleotide polymorphism within eukaryotic peptide chain release factor (eRF1), which participates in stop-codon recognition and nascent-polypeptide release from ribosomes during translation. A subsequent complementation test revealed that ESP1 encodes eRF1. We also identified UAA as the stop codon of CysP prolamines with reduced concentration in esp1 mutants. Recognition assays and microarray analysis confirmed that ESP1/eRF1 recognizes UAA/UAG, but not UGA. Our results provide convincing evidence that ESP1/eRF1 participates in the translation termination of CysP prolamines during seed development.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalPlant Science
Volume281
DOIs
Publication statusPublished - Apr 1 2019

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Phenylpropanolamine
Endosperm
prolamins
translation (genetics)
endosperm
Cysteine
cysteine
peptides
rice
Peptides
Terminator Codon
stop codon
alcohols
Alcohols
Genetic Complementation Test
mutants
Genetic Linkage
Chromosomes, Human, Pair 7
reducing agents
Reducing Agents

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Eukaryotic peptide chain release factor 1 participates in translation termination of specific cysteine-poor prolamines in rice endosperm. / Elakhdar, Ammar; Ushijima, Tomokazu; Fukuda, Masako; Yamashiro, Noriko; Kawagoe, Yasushi; Kumamaru, Toshihiro.

In: Plant Science, Vol. 281, 01.04.2019, p. 223-231.

Research output: Contribution to journalArticle

Elakhdar, Ammar ; Ushijima, Tomokazu ; Fukuda, Masako ; Yamashiro, Noriko ; Kawagoe, Yasushi ; Kumamaru, Toshihiro. / Eukaryotic peptide chain release factor 1 participates in translation termination of specific cysteine-poor prolamines in rice endosperm. In: Plant Science. 2019 ; Vol. 281. pp. 223-231.
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