Evaluation of α-glucosidase inhibition by using an immobilized assay system

T. Oki, Toshiro Matsui, K. Matsumoto

Research output: Contribution to journalArticle

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Abstract

The inhibitory effects of natural and synthetic inhibitors on the intestinal membrane-bound hydrolase, α-glucosidase (AGH), were evaluated by using an immobilized cyanogen bromide-activated Sepharose 4B support. Immobilized AGH (iAGH) inhibition study by synthetic inhibitors (acarbose and voglibose) revealed that the magnitude of inhibition differed from that in the free AGH (fAGH) study: IC50 value of acarbose in iAGH-maltase assay system, 340 - 430 nM; fAGH, 11 nM. iAGH-maltase inhibition by both inhibitors was influenced by blocking reagents with different functional groups (COOH, OH, CH3, and NH2 groups). On the other hand, significant iAGH-sucrase inhibitory activity was observed only when using the negatively charged support induced by 0.1 M β-alanine. The K(m) values obtained in the iAGH assay system were similar to those from the fAGH method. With natural inhibitors, the iAGH-sucrase inhibitory activity of D-xylose, with in vivo glucose suppression, increased twice compared to that in fAGH. Green tea extract gave almost the same inhibition for both AGH assay systems.

Original languageEnglish
Pages (from-to)1084-1087
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number9
DOIs
Publication statusPublished - Jan 1 2000

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Acarbose
Glucosidases
Sucrase
alpha-Glucosidases
Cyanogen Bromide
Xylose
Hydrolases
Tea
Alanine
Sepharose
Inhibitory Concentration 50
Glucose
Membranes
voglibose

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Evaluation of α-glucosidase inhibition by using an immobilized assay system. / Oki, T.; Matsui, Toshiro; Matsumoto, K.

In: Biological and Pharmaceutical Bulletin, Vol. 23, No. 9, 01.01.2000, p. 1084-1087.

Research output: Contribution to journalArticle

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