Evaluation of a dysfunctional and short-lived subset of monocyte-derived dendritic cells from cancer patients

Hideya Onishi, Takashi Morisaki, Hideo Kuroki, Kotaro Matsumoto, Eishi Baba, Hirotaka Kuga, Masao Tanaka, Mitsuo Katano

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5 Citations (Scopus)

Abstract

Monocyte-derived dendritic cells (Mo-DCs) were generated from peripheral blood monocytes of 12 healthy volunteers (hMo-DCs) and 11 patients (pMo-DCs) with malignancies by culture for 7 days with granulocyte-macrophage colony-stimulating factor and interleukin-4. In this study, we focused on the cytogram pattern by FACS analysis. A gate (R1) was set up by which more than 95% of hMo-DCs were contained. Mo-DCs having lower side scatter than the R1 (R2) comprised 4.5% of hMo-DCs and 24.2% of pMo-DCs. Expressions of antigen presentation-related molecules and phagocytic ability in the R2 of pMo-DCs were lower than those in the R1 population. The R2, but not R1, in pMo-DCs decreased in number between days 7 and 14, and expression levels of antigen presentation-related molecules in the living pMo-DCs on day 14 increased. The 11 patients received dendritic cell vaccine therapy with autologous, tumor-pulsed mature Mo-DCs (day 7). The low R2 group (R2<10%, 3 patients) had a significantly higher positive delayed-type hypersensitivity reaction against autologous tumor-pulsed Mo-DCs than that of the high R2 group (R2>10%, 8 patients) (p<0.001). These results indicate that the R2 of pMo-DCs may be a dysfunctional and short-lived subset.

Original languageEnglish
Pages (from-to)3445-3451
Number of pages7
JournalAnticancer research
Volume25
Issue number5
Publication statusPublished - Sep 1 2005

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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