TY - JOUR
T1 - Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts
AU - Morinaga, Osamu
AU - Uto, Takuhiro
AU - Yuan, Chun Su
AU - Tanaka, Hiroyuki
AU - Shoyama, Yukihiro
N1 - Funding Information:
The authors thank Dr. Thomas S.C. Li (Agriculture and Agri-Food Canada) for supplying the American ginseng berry pulp samples and his valuable suggestions. This research was supported in part by a Grant-in-Aid (No. 21790030 for O. M.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan , the research fund of Japan Society for the Promotion of Science (JSPS Asian Core Program) , and Takeda Science Foundation .
PY - 2010/6
Y1 - 2010/6
N2 - A new eastern blotting technique has been established for ginsenoside Re (G-Re) contained in American ginseng berry pulp extracts. G-Re in American ginseng berry pulp was extracted using 100% methanol, 100% ethanol, 50% aqueous methanol, and 50% aqueous ethanol. The combined crude extracts were applied onto a polyethersulfone membrane and developed using the methanol-water-acetic acid solvent system (45:55:1 v/v). Separated components were immunostained using anti-G-Re monoclonal antibody. G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp.
AB - A new eastern blotting technique has been established for ginsenoside Re (G-Re) contained in American ginseng berry pulp extracts. G-Re in American ginseng berry pulp was extracted using 100% methanol, 100% ethanol, 50% aqueous methanol, and 50% aqueous ethanol. The combined crude extracts were applied onto a polyethersulfone membrane and developed using the methanol-water-acetic acid solvent system (45:55:1 v/v). Separated components were immunostained using anti-G-Re monoclonal antibody. G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp.
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U2 - 10.1016/j.fitote.2009.10.005
DO - 10.1016/j.fitote.2009.10.005
M3 - Article
C2 - 19850112
AN - SCOPUS:77950518981
SN - 0367-326X
VL - 81
SP - 284
EP - 288
JO - Fitoterapia
JF - Fitoterapia
IS - 4
ER -