Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts

Osamu Morinaga, Takuhiro Uto, Chun Su Yuan, Hiroyuki Tanaka, Yukihiro Shoyama

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11 Citations (Scopus)

Abstract

A new eastern blotting technique has been established for ginsenoside Re (G-Re) contained in American ginseng berry pulp extracts. G-Re in American ginseng berry pulp was extracted using 100% methanol, 100% ethanol, 50% aqueous methanol, and 50% aqueous ethanol. The combined crude extracts were applied onto a polyethersulfone membrane and developed using the methanol-water-acetic acid solvent system (45:55:1 v/v). Separated components were immunostained using anti-G-Re monoclonal antibody. G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalFitoterapia
Volume81
Issue number4
DOIs
Publication statusPublished - Jun 1 2010

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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