Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts

Osamu Morinaga, Takuhiro Uto, Chun Su Yuan, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new eastern blotting technique has been established for ginsenoside Re (G-Re) contained in American ginseng berry pulp extracts. G-Re in American ginseng berry pulp was extracted using 100% methanol, 100% ethanol, 50% aqueous methanol, and 50% aqueous ethanol. The combined crude extracts were applied onto a polyethersulfone membrane and developed using the methanol-water-acetic acid solvent system (45:55:1 v/v). Separated components were immunostained using anti-G-Re monoclonal antibody. G-Re was first specifically detected and then quantitatively analyzed using NIH Imaging software. We also confirmed that the most suitable solvent was 50% aqueous methanol for extracting G-Re from American ginseng berry pulp.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalFitoterapia
Volume81
Issue number4
DOIs
Publication statusPublished - Jun 1 2010

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Panax
Fruit
Methanol
Ethanol
Complex Mixtures
Acetic Acid
Software
Monoclonal Antibodies
ginsenoside Re
Membranes
Water

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts. / Morinaga, Osamu; Uto, Takuhiro; Yuan, Chun Su; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Fitoterapia, Vol. 81, No. 4, 01.06.2010, p. 284-288.

Research output: Contribution to journalArticle

Morinaga, Osamu ; Uto, Takuhiro ; Yuan, Chun Su ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts. In: Fitoterapia. 2010 ; Vol. 81, No. 4. pp. 284-288.
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