TY - JOUR
T1 - Evaluation of change in metabolome caused by comprehensive diabetes treatment
T2 - A prospective observational study of diabetes inpatients with gas chromatography/mass spectrometry-based non-target metabolomic analysis
AU - Taya, Naohiro
AU - Katakami, Naoto
AU - Omori, Kazuo
AU - Arakawa, Shoya
AU - Hosoe, Shigero
AU - Watanabe, Hirotaka
AU - Takahara, Mitsuyoshi
AU - Miyashita, Kazuyuki
AU - Nishizawa, Hitoshi
AU - Matsuoka, Taka Aki
AU - Furuno, Masahiro
AU - Bamba, Takeshi
AU - Iida, Junko
AU - Fukusaki, Eiichiro
AU - Shimomura, Iichiro
N1 - Funding Information:
The authors thank study participants for their participation. This study was supported by AMED-CREST, Japan Agency for Medical Research and Development (AMED; grant number: JP19gm0710005).
Funding Information:
The authors thank study participants for their participation. This study was supported by AMED‐CREST, Japan Agency for Medical Research and Development (AMED; grant number: JP19gm0710005).
Publisher Copyright:
© 2021 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd
PY - 2021/12
Y1 - 2021/12
N2 - Aims/Introduction: Diabetes patients develop a variety of metabolic abnormalities in addition to hyperglycemia. However, details regarding change in various metabolites after comprehensive diabetes treatment remain unknown. This study aimed to identify the short-term change in metabolome in inpatients who were subject to comprehensive diabetes treatment, using gas chromatography/mass spectrometry-based non-target metabolomics techniques. Materials and Methods: Participants of the present study were randomly recruited from the patients with type 2 diabetes hospitalized due to problems with glycemic control (n = 31) and volunteers without diabetes (n = 30), both of whom were aged between 20 and 75 years. A metabolomic analysis of fasting plasma samples on the 2nd (pre-treatment) and 16th hospital (post-treatment) day with gas chromatography/mass spectrometry using a multiple reaction monitoring mode was carried out. Results: A principal component analysis showed that metabolome of fasting plasma was different between individuals with and without diabetes. The metabolome of fasting plasma in diabetes patients after treatment was different from that of pre-treatment, as well as individuals without diabetes. Many amino acids (proline, glycine, serine, threonine, methionine, pyroglutamic acid, glutamine and lysine) were significantly increased by >10% after administering the inpatient diabetes treatment. A hierarchical clustering analysis showed that in the case of patients with markedly decreased monosaccharide levels and increased 1,5-anhydroglucitol, the levels of amino acids increased more significantly. Conclusions: After a 2-week comprehensive treatment, the plasma levels of various amino acids increased in conjunction with the reduction in monosaccharide levels in poorly controlled type 2 diabetes patients.
AB - Aims/Introduction: Diabetes patients develop a variety of metabolic abnormalities in addition to hyperglycemia. However, details regarding change in various metabolites after comprehensive diabetes treatment remain unknown. This study aimed to identify the short-term change in metabolome in inpatients who were subject to comprehensive diabetes treatment, using gas chromatography/mass spectrometry-based non-target metabolomics techniques. Materials and Methods: Participants of the present study were randomly recruited from the patients with type 2 diabetes hospitalized due to problems with glycemic control (n = 31) and volunteers without diabetes (n = 30), both of whom were aged between 20 and 75 years. A metabolomic analysis of fasting plasma samples on the 2nd (pre-treatment) and 16th hospital (post-treatment) day with gas chromatography/mass spectrometry using a multiple reaction monitoring mode was carried out. Results: A principal component analysis showed that metabolome of fasting plasma was different between individuals with and without diabetes. The metabolome of fasting plasma in diabetes patients after treatment was different from that of pre-treatment, as well as individuals without diabetes. Many amino acids (proline, glycine, serine, threonine, methionine, pyroglutamic acid, glutamine and lysine) were significantly increased by >10% after administering the inpatient diabetes treatment. A hierarchical clustering analysis showed that in the case of patients with markedly decreased monosaccharide levels and increased 1,5-anhydroglucitol, the levels of amino acids increased more significantly. Conclusions: After a 2-week comprehensive treatment, the plasma levels of various amino acids increased in conjunction with the reduction in monosaccharide levels in poorly controlled type 2 diabetes patients.
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U2 - 10.1111/jdi.13600
DO - 10.1111/jdi.13600
M3 - Article
C2 - 34032389
AN - SCOPUS:85115775840
VL - 12
SP - 2232
EP - 2241
JO - Journal of Diabetes Investigation
JF - Journal of Diabetes Investigation
SN - 2040-1116
IS - 12
ER -
