Evaluation of intestinal microbiotas of healthy Japanese adults and effect of antibiotics using the 16S ribosomal RNA gene based clone library method

Nobuo Morotomi, Kazumasa Fukuda, Masahiro Nakano, Sachiko Ichihara, Taichi Oono, Taiga Yamazaki, Noritada Kobayashi, Tatsuo Suzuki, Yoshiya Tanaka, Hatsumi Taniguchi

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Abstract

Intestinal microbiotas of human subjects and effect of antibiotic treatment on them have been reported with cultivation independent methods. However, Japanese fecal microbiotas have not been studied enough.We have constructed a clone library method to obtain results within 3 d. In this study, intestinal microbiotas of 29 healthy Japanese adults, whose fecal samples were collected twice at 5 month intervals from each subject, were analyzed with our clone library method, and using those data as a benchmark effect of antibiotic treatment on intestinal microbiotas was evaluated. The fifty-eight fecal microbiotas were assessed based on percentages at genus level, and the variability was analyzed with a principal component analysis (PCA). PCA showed that the microbiotas divided into three groups depending on the large eigenvectors (genera Ruminococcus, Bacteroides, and Prevotella), and the dual samples from the twenty-two individuals have belonged to the same PCA group. It suggests that almost Japanese adults have own stable intestinal microbiota. The genera Ruminococcus and Bacteroides were present in almost subjects, while the genus Prevotella was found only in nine subjects (approximately 30%) which was preserved with 5 months intervals. Next, the microbiotas before and after antibiotic treatment were evaluated comparing with the 58 healthy adult microbiotas. The results showed that beta-lactams influenced pro-foundly on intestinal microbiotas and the effect of macrolides depended on the cases. It suggests that our clone library method could show overview of intestinal microbiota and would give us useful information about the effect of antibiotic treatment for daily clinical diagnosis.

Original languageEnglish
Pages (from-to)1011-1020
Number of pages10
JournalBiological and Pharmaceutical Bulletin
Volume34
Issue number7
DOIs
Publication statusPublished - Jul 1 2011

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16S Ribosomal RNA
rRNA Genes
Microbiota
Clone Cells
Anti-Bacterial Agents
Ruminococcus
Principal Component Analysis
Prevotella
Bacteroides
Benchmarking
Macrolides
beta-Lactams
Therapeutics
Gastrointestinal Microbiome

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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Evaluation of intestinal microbiotas of healthy Japanese adults and effect of antibiotics using the 16S ribosomal RNA gene based clone library method. / Morotomi, Nobuo; Fukuda, Kazumasa; Nakano, Masahiro; Ichihara, Sachiko; Oono, Taichi; Yamazaki, Taiga; Kobayashi, Noritada; Suzuki, Tatsuo; Tanaka, Yoshiya; Taniguchi, Hatsumi.

In: Biological and Pharmaceutical Bulletin, Vol. 34, No. 7, 01.07.2011, p. 1011-1020.

Research output: Contribution to journalArticle

Morotomi, Nobuo ; Fukuda, Kazumasa ; Nakano, Masahiro ; Ichihara, Sachiko ; Oono, Taichi ; Yamazaki, Taiga ; Kobayashi, Noritada ; Suzuki, Tatsuo ; Tanaka, Yoshiya ; Taniguchi, Hatsumi. / Evaluation of intestinal microbiotas of healthy Japanese adults and effect of antibiotics using the 16S ribosomal RNA gene based clone library method. In: Biological and Pharmaceutical Bulletin. 2011 ; Vol. 34, No. 7. pp. 1011-1020.
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