Evaluation of polyanion-coated biodegradable polymeric micelles as drug delivery vehicles

Yuichi Ohya, Shinya Takeda, Yosuke Shibata, Tatsuro Ouchi, Arihiro Kano, Tomoki Iwata, Shinichi Mochizuki, Yuki Taniwaki, Atsushi Maruyama

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Polymeric micelles, as drug delivery vehicles, must achieve specific targeting and high stability in the body for efficient drug delivery. We recently reported the preparation of polyanion-coated biodegradable polymeric micelles by coating positively charged polymeric micelles consisting of poly(l-lysine)-block-poly(l-lactide) (PLys-b-PLLA) AB diblock copolymers with anionic hyaluronic acid (HA) by polyion complex (PIC) formation. The obtained HA-coated micelles showed significantly higher stability in aqueous solution. In this study, to evaluate the HA-coated polymeric micelles as a drug carrier, model drug release from the micelles and cytotoxicity of the micelles were investigated. The HA-coated micelles showed sustained release of model drugs and low cytotoxicity. It is known that there are receptors for HA on liver sinusoidal endothelial cells (LSEC). Specific interactions of HA-coated micelles with LSECs and Kupffer cells were investigated and compared with polymeric micelles coated with other polyanionic polysaccharides, i.e., heparin (Hep) and carboxymethyl-dextran (CMDex). Although Hep-coated micelles and CMDex-coated micelles were incorporated into both Kupffer cells and LSECs, HA-coated micelles were taken up only into LSECs. These results suggest HA-coated micelles have potential utility as drug delivery vehicles exhibiting specific accumulation into LSECs.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalJournal of Controlled Release
Volume155
Issue number1
DOIs
Publication statusPublished - Oct 10 2011

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Micelles
Hyaluronic Acid
Pharmaceutical Preparations
Kupffer Cells
polyanions
Heparin
Drug Carriers
Lysine
Polysaccharides
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Evaluation of polyanion-coated biodegradable polymeric micelles as drug delivery vehicles. / Ohya, Yuichi; Takeda, Shinya; Shibata, Yosuke; Ouchi, Tatsuro; Kano, Arihiro; Iwata, Tomoki; Mochizuki, Shinichi; Taniwaki, Yuki; Maruyama, Atsushi.

In: Journal of Controlled Release, Vol. 155, No. 1, 10.10.2011, p. 104-110.

Research output: Contribution to journalArticle

Ohya, Y, Takeda, S, Shibata, Y, Ouchi, T, Kano, A, Iwata, T, Mochizuki, S, Taniwaki, Y & Maruyama, A 2011, 'Evaluation of polyanion-coated biodegradable polymeric micelles as drug delivery vehicles', Journal of Controlled Release, vol. 155, no. 1, pp. 104-110. https://doi.org/10.1016/j.jconrel.2010.11.008
Ohya, Yuichi ; Takeda, Shinya ; Shibata, Yosuke ; Ouchi, Tatsuro ; Kano, Arihiro ; Iwata, Tomoki ; Mochizuki, Shinichi ; Taniwaki, Yuki ; Maruyama, Atsushi. / Evaluation of polyanion-coated biodegradable polymeric micelles as drug delivery vehicles. In: Journal of Controlled Release. 2011 ; Vol. 155, No. 1. pp. 104-110.
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