Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit

Landon J. Inge, Sigrid A. Rajasekaran, Koji Yoshimoto, Paul S. Mischel, William McBride, Elliot Landaw, Ayyappan K. Rajasekaran

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Na,K-ATPase, consisting of two essential subunits (a, ß), plays a critical role in the regulation of ion homeostasis in mammalian cells. Recent studies indicate that reduced expression of the ß1 isoform (NaK-ß1) is commonly observed in carcinoma and is associated with events involved in cancer progression. In this study, we present evidence that repletion of NaK-ß1 in Moloney sarcoma virustransformed Madin-Darby canine kidney cells (MSVMDCK), a highly tumorigenic cell line, inhibits anchorage independent growth and suppresses tumor formation in immunocompromised mice. Additionally, using an in vitro cell-cell aggregation assay, we showed that cell aggregates of NaK-ß1 subunit expressing MSVMDCK cells have reduced extracellular regulated kinase (ERK) 1/2 activity compared with parental MSV-MDCK cells. Finally, using immunohistochemistry and fully quantitative image analysis approaches, we showed that the levels of phosphorylated ERK 1/2 are inversely correlated to the NaK-ß1 levels in the tumors. These findings reveal for the first time that NaK-ß1 has a potential tumor-suppressor function in epithelial cells.

Original languageEnglish
Pages (from-to)459-467
Number of pages9
JournalHistology and Histopathology
Volume23
Issue number4
Publication statusPublished - Apr 2008
Externally publishedYes

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Madin Darby Canine Kidney Cells
Sarcoma
Neoplasms
Phosphotransferases
Cell Aggregation
Protein Isoforms
Homeostasis
Epithelial Cells
Immunohistochemistry
Ions
Carcinoma
Cell Line
sodium-translocating ATPase
Growth

All Science Journal Classification (ASJC) codes

  • Histology
  • Pathology and Forensic Medicine

Cite this

Inge, L. J., Rajasekaran, S. A., Yoshimoto, K., Mischel, P. S., McBride, W., Landaw, E., & Rajasekaran, A. K. (2008). Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit. Histology and Histopathology, 23(4), 459-467.

Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit. / Inge, Landon J.; Rajasekaran, Sigrid A.; Yoshimoto, Koji; Mischel, Paul S.; McBride, William; Landaw, Elliot; Rajasekaran, Ayyappan K.

In: Histology and Histopathology, Vol. 23, No. 4, 04.2008, p. 459-467.

Research output: Contribution to journalArticle

Inge, LJ, Rajasekaran, SA, Yoshimoto, K, Mischel, PS, McBride, W, Landaw, E & Rajasekaran, AK 2008, 'Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit', Histology and Histopathology, vol. 23, no. 4, pp. 459-467.
Inge LJ, Rajasekaran SA, Yoshimoto K, Mischel PS, McBride W, Landaw E et al. Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit. Histology and Histopathology. 2008 Apr;23(4):459-467.
Inge, Landon J. ; Rajasekaran, Sigrid A. ; Yoshimoto, Koji ; Mischel, Paul S. ; McBride, William ; Landaw, Elliot ; Rajasekaran, Ayyappan K. / Evidence for a potential tumor suppressor role for the Na,K-ATPase ß1-subunit. In: Histology and Histopathology. 2008 ; Vol. 23, No. 4. pp. 459-467.
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