Evidence for an origin of ethyl-nitrosourea-induced rat central nervous system tumors from pluripotent germinal neuroepithelium

M. Shibutani, K. Mitsumori, R. Okeda, T. Imazawa, A. Maekawa, H. Hatanaka, M. Takahashi, Kazuhide Inoue, Y. Hayashi

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Abstract

Brain tumors induced by transplacental application of ethyl-nitrosourea (ENU) in F344 rats were immunohistochemically demonstrated to consist of undifferentiated cells, astriocyte-like cells, oligodendroglia-like cells, and two distinct types of vimentin-expressing cell groupings termed as perivascular small cell nests (PSCNs) and large cell nests (LCNs). Co-distribution of vimentin and glial fibrillary acidic protein (GFAP) was sparsely ovserved in the astrocyte-like cells, which suggested an immature glial phenotype. PCSNs contained cells expressing GFAP, neuron-specific enolase (NSE), β-tubulin isotype III, and low-affinity nerve growth factor receptors (LNGFRs). LCNs contained cells showing a neuronal phenotype with expression of low- and middle-molecular mass neurofilament proteins (NF-L and-M) as well as NSE, β-tubulin isotype III and LNGFR. Double-labelling immunohistochemistry revealed the NF-L-expression in LNGFR-positive LCN-forming cells. Oligodendroglia-like cells and their intercellular neuropil-like structures expressed β-tubulin isotype III, synaptophysin and NSE, in addition to the expressions of vimentin and GFAP. Electron microscopically, synapse-like structures were formed between these oligodendroglia-like cells and their dendritic processes. Topographically, bidirectional cell transitions from PSCNs to astrocytes and LCNs were indicated. The present study strongly suggests that so-called ENU-induced "gliomas" originate from pluripotent germinal neuroepithelium. Furthermore, LNGFR expression may be responsible for acquisition of neuronal phenotype in these tumors.

Original languageEnglish
Pages (from-to)293-301
Number of pages9
JournalActa Neuropathologica
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 1994
Externally publishedYes

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Central Nervous System Neoplasms
Nerve Growth Factor Receptor
Phosphopyruvate Hydratase
Glial Fibrillary Acidic Protein
Oligodendroglia
Vimentin
Tubulin
Phenotype
Astrocytes
Synaptophysin
Neuropil
Inbred F344 Rats

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Shibutani, M., Mitsumori, K., Okeda, R., Imazawa, T., Maekawa, A., Hatanaka, H., ... Hayashi, Y. (1994). Evidence for an origin of ethyl-nitrosourea-induced rat central nervous system tumors from pluripotent germinal neuroepithelium. Acta Neuropathologica, 87(3), 293-301. https://doi.org/10.1007/BF00296745

Evidence for an origin of ethyl-nitrosourea-induced rat central nervous system tumors from pluripotent germinal neuroepithelium. / Shibutani, M.; Mitsumori, K.; Okeda, R.; Imazawa, T.; Maekawa, A.; Hatanaka, H.; Takahashi, M.; Inoue, Kazuhide; Hayashi, Y.

In: Acta Neuropathologica, Vol. 87, No. 3, 03.1994, p. 293-301.

Research output: Contribution to journalArticle

Shibutani, M, Mitsumori, K, Okeda, R, Imazawa, T, Maekawa, A, Hatanaka, H, Takahashi, M, Inoue, K & Hayashi, Y 1994, 'Evidence for an origin of ethyl-nitrosourea-induced rat central nervous system tumors from pluripotent germinal neuroepithelium', Acta Neuropathologica, vol. 87, no. 3, pp. 293-301. https://doi.org/10.1007/BF00296745
Shibutani, M. ; Mitsumori, K. ; Okeda, R. ; Imazawa, T. ; Maekawa, A. ; Hatanaka, H. ; Takahashi, M. ; Inoue, Kazuhide ; Hayashi, Y. / Evidence for an origin of ethyl-nitrosourea-induced rat central nervous system tumors from pluripotent germinal neuroepithelium. In: Acta Neuropathologica. 1994 ; Vol. 87, No. 3. pp. 293-301.
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