Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis

Y. Kimura, S. Tomida, Y. Matsumoto, K. Hiromatsu, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    We have previously reported that heat-shock protein (hsp) 60-reactive T-cell receptor (TCR)γδ + T cells appear in the peritoneal cavity during the early stage of infection with Listeria monocytogenes in mice. In this study, we examined the kinetics of TCRγδ + T cells during listeriosis in F344 rats by flow cytometry using a V65 monoclonal antibody (mAb) directed to a constant determinant of rat TCRγδ chains. TCRγδ + T cells significantly increased in the peritoneal cavity on day 6 and then decreased by day 10 after infection, in parallel with the kinetics of hsp60 expression in the peritoneal macrophages during listeriosis in F344 rats. Most of the early appearing TCRγδ + T cells were of the CD4 - CD8αβ + CD5 + lymphocyte function-associated antigen (LFA)-1α(high) CD45RC - interleukin-2 receptor (IL-2R) α - phenotype, although a significant fraction of the TCRγδ + T cells expressed CD8α only. The increase in TCRγδ + T cells during listeriosis was prominent in F 1 (F344 x Lewis) rats but only marginal in Lewis rats, which was correlated with the expression level of hsp 60 in the peritoneal macrophages. The peritoneal TCRγδ + T cells in naive F344 rats appeared to proliferate significantly in response to recombinant hsp 60 (rhsp 60) derived from Mycobacterium bovis bacillus Calmette-Guerin (BCG). These results imply that the early appearance of hsp 60-reactive TCRγδ + T cells during listerial infection can be generalized across species.

    Original languageEnglish
    Pages (from-to)21-28
    Number of pages8
    JournalImmunology
    Volume87
    Issue number1
    Publication statusPublished - Jan 23 1996

    Fingerprint

    Listeriosis
    T-Cell Antigen Receptor
    T-Lymphocytes
    Chaperonin 60
    Inbred F344 Rats
    Peritoneal Cavity
    Peritoneal Macrophages
    Mycobacterium bovis
    Infection
    Lymphocyte Function-Associated Antigen-1
    Interleukin-2 Receptors
    Listeria monocytogenes
    Recombinant Proteins
    Flow Cytometry
    Monoclonal Antibodies
    Phenotype

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Kimura, Y., Tomida, S., Matsumoto, Y., Hiromatsu, K., & Yoshikai, Y. (1996). Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis Immunology, 87(1), 21-28.

    Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis . / Kimura, Y.; Tomida, S.; Matsumoto, Y.; Hiromatsu, K.; Yoshikai, Yasunobu.

    In: Immunology, Vol. 87, No. 1, 23.01.1996, p. 21-28.

    Research output: Contribution to journalArticle

    Kimura, Y, Tomida, S, Matsumoto, Y, Hiromatsu, K & Yoshikai, Y 1996, ' Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis ', Immunology, vol. 87, no. 1, pp. 21-28.
    Kimura Y, Tomida S, Matsumoto Y, Hiromatsu K, Yoshikai Y. Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis Immunology. 1996 Jan 23;87(1):21-28.
    Kimura, Y. ; Tomida, S. ; Matsumoto, Y. ; Hiromatsu, K. ; Yoshikai, Yasunobu. / Evidence for the early recruitment of T-cell receptor γδ + T cells during rat listeriosis In: Immunology. 1996 ; Vol. 87, No. 1. pp. 21-28.
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