Evidence of B cell clonal expansion in HIV type 1-infected patients

Y. Chong, H. Ikematsu, I. Ariyama, K. Chijiwa, W. Li, K. Yamaji, S. Kashiwagi, J. Hayashi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

HIV-1 infection results in a gradual decrease in CD4+ T cell counts and progressive immune deficiency. Increased T cell turnover in HIV-1-infected patients, which can be interpreted as T cell clonal expansion, has been thought to be relevant to its pathogenesis. To investigate whether B cell clonal expansion also occurs in HIV-1-infected patients, we examined the expressed VHDJH gene sequences of peripheral B cells in HIV-1-infected patients with hypergammaglobulinemia. Identical VHDJH gene rearrangements with additional nucleotide differences in VH genes were analyzed as a marker of clonally related B cells. From healthy individuals and HIV-1-uninfected patients with hypergammaglobulinemia, clonally related B cells were detected in none of 10 (0%) and 2 of 10 (20%), respectively. No clonally related B cells were detected in any of the nine HIV-1-infected patients with detectable viral loads and normal Ig levels (0%). In contrast, from 9 of 14 HIV-1-infected patients with hypergammaglobulinemia (64%), clonally related B cells were detected. In addition, no HIV-1-infected patients who exhibited normal Ig levels after antiretroviral therapy had clonally related B cells. These findings suggest that B cell clonal expansion is present in HIV-1-infected patients with hypergammaglobulinemia.

Original languageEnglish
Pages (from-to)1507-1515
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume17
Issue number16
DOIs
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Infectious Diseases

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