Evidence of cyanamide production in hairy vetch Vicia villosa

Tsunashi Kamo, Kenji Kato, Syuntaro Hiradate, Eri Nakajima, Yoshiharu Fujii, Mitsuru Hirota

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4 Citations (Scopus)

Abstract

Cyanamide (NH2CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4mM potassium (15N)nitrate was administered to 15- to 35-day-old plants of V. villosa, from which the cyanamide was purified and subjected to GC/MS analysis. The isotopic ratio 15N/(14 N+15N) of the cyanamide was calculated to be 0.143, while that of the cyanamide extracted from V. villosa grown in the presence of a natural N source was 0.0065. The 15N-enrichment proved de novo biosynthesis of cyanamide.

Original languageEnglish
Pages (from-to)429-433
Number of pages5
JournalNatural Product Research
Volume20
Issue number5
DOIs
Publication statusPublished - May 10 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

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