TY - JOUR
T1 - Evidence of cyanamide production in hairy vetch Vicia villosa
AU - Kamo, Tsunashi
AU - Kato, Kenji
AU - Hiradate, Syuntaro
AU - Nakajima, Eri
AU - Fujii, Yoshiharu
AU - Hirota, Mitsuru
N1 - Funding Information:
This work was supported in part by grant aid from Japan Society for the Promotion of Science (16780082 to T. K.).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2006/5/10
Y1 - 2006/5/10
N2 - Cyanamide (NH2CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4mM potassium (15N)nitrate was administered to 15- to 35-day-old plants of V. villosa, from which the cyanamide was purified and subjected to GC/MS analysis. The isotopic ratio 15N/(14 N+15N) of the cyanamide was calculated to be 0.143, while that of the cyanamide extracted from V. villosa grown in the presence of a natural N source was 0.0065. The 15N-enrichment proved de novo biosynthesis of cyanamide.
AB - Cyanamide (NH2CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4mM potassium (15N)nitrate was administered to 15- to 35-day-old plants of V. villosa, from which the cyanamide was purified and subjected to GC/MS analysis. The isotopic ratio 15N/(14 N+15N) of the cyanamide was calculated to be 0.143, while that of the cyanamide extracted from V. villosa grown in the presence of a natural N source was 0.0065. The 15N-enrichment proved de novo biosynthesis of cyanamide.
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U2 - 10.1080/14786410500143583
DO - 10.1080/14786410500143583
M3 - Article
C2 - 16644540
AN - SCOPUS:33646410698
SN - 1478-6419
VL - 20
SP - 429
EP - 433
JO - Natural Product Research
JF - Natural Product Research
IS - 5
ER -