Evidence that angiotensin II is present in human monocytes

Takanari Kitazono, Richard C. Padgett, Mark L. Armstrong, Pamela K. Tompkins, Donald D. Heistad

Research output: Contribution to journalArticle

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Abstract

Background: The purpose of the present study was to determine whether human monocytes and polymorphonuclear leukocytes contain angiotensins I and II. Methods and Results: Human mononuclear and polymorphonuclear leukocytes were isolated from blood. To identify angiotensins in human leukocytes, we performed immunocytochemistry using both alkaline phosphatase and fluorescence methods. With light microscopy immunocytochemistry with akaline phosphatase, prominent staining of angiotensin II was observed in mononuclear leukocytes. Angiotensin I was also demonstrated in mononuclear leukocytes, but the signal was less pronounced than for angiotensin II. Polymorphonuclear leukocytes showed very little staining for angiotensin II. Fluorescence immunocytochemistry also demonstrated angiotensin II in mononuclear leukocytes. Angiotensins I and II in homogenate of leukocytes were quantified by radioimmunoassay. The concentration of angiotensins I and II in mononuclear leukocytes was 355±216 (mean±SEM) and 2331±106 fmol/mg protein, respectively, and the concentration in polymorphonuclear leukocytes was 36±10 and 336±120 fmol/mg protein. Conclusions: These findings suggest that human mononuclear leukocytes contain large amounts of angiotensin II and lesser amounts of angiotensin I. Human polymorphonuclear leukocytes contain small amounts of angiotensin I and II.

Original languageEnglish
Pages (from-to)1129-1134
Number of pages6
JournalCirculation
Volume91
Issue number4
DOIs
Publication statusPublished - Feb 15 1995
Externally publishedYes

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Angiotensin II
Mononuclear Leukocytes
Monocytes
Angiotensin I
Neutrophils
Immunohistochemistry
Leukocytes
Fluorescence
Staining and Labeling
Angiotensins
Phosphoric Monoester Hydrolases
Radioimmunoassay
Alkaline Phosphatase
Microscopy
Proteins
Light

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Kitazono, T., Padgett, R. C., Armstrong, M. L., Tompkins, P. K., & Heistad, D. D. (1995). Evidence that angiotensin II is present in human monocytes. Circulation, 91(4), 1129-1134. https://doi.org/10.1161/01.CIR.91.4.1129

Evidence that angiotensin II is present in human monocytes. / Kitazono, Takanari; Padgett, Richard C.; Armstrong, Mark L.; Tompkins, Pamela K.; Heistad, Donald D.

In: Circulation, Vol. 91, No. 4, 15.02.1995, p. 1129-1134.

Research output: Contribution to journalArticle

Kitazono, T, Padgett, RC, Armstrong, ML, Tompkins, PK & Heistad, DD 1995, 'Evidence that angiotensin II is present in human monocytes', Circulation, vol. 91, no. 4, pp. 1129-1134. https://doi.org/10.1161/01.CIR.91.4.1129
Kitazono T, Padgett RC, Armstrong ML, Tompkins PK, Heistad DD. Evidence that angiotensin II is present in human monocytes. Circulation. 1995 Feb 15;91(4):1129-1134. https://doi.org/10.1161/01.CIR.91.4.1129
Kitazono, Takanari ; Padgett, Richard C. ; Armstrong, Mark L. ; Tompkins, Pamela K. ; Heistad, Donald D. / Evidence that angiotensin II is present in human monocytes. In: Circulation. 1995 ; Vol. 91, No. 4. pp. 1129-1134.
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