Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury

Tomoharu Yoshizumi, Yasuhiro Ikeda, Y. Kaneda, K. Sueishi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. Specific inhibition of these cytokines may improve viability of the hepatic graft upon reperfusion. Herein we have assessed the efficacy of cis element decoy against nuclear factor-κB binding site delivery to the hepatic tissue in a rodent liver transplantation model. At 8 hours after reperfusion of the liver, significant reduction was noted in the livers treated with decoy in the release of cytosolic enzymes from the hepatocytes and in serum tumor necrosis factor α (P < .05). The neutrophilic infiltration into the hepatic grafts was significantly suppressed in the livers treated with decoy oligodeoxynucleotides (ODNs). Decoy ODNs against nuclear factor-κB binding site delivery improved the viability of the hepatic graft against cold ischemia/reperfusion injury in the rodent liver transplantation model.

Original languageEnglish
Pages (from-to)1504-1507
Number of pages4
JournalTransplantation Proceedings
Volume41
Issue number5
DOIs
Publication statusPublished - Jun 1 2009

Fingerprint

Cold Ischemia
Transfer Factor
Reperfusion Injury
Liver
Transplants
Oligodeoxyribonucleotides
Liver Transplantation
Reperfusion
Rodentia
Binding Sites
Cytokines
Hepatocytes
Tumor Necrosis Factor-alpha
Enzymes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury. / Yoshizumi, Tomoharu; Ikeda, Yasuhiro; Kaneda, Y.; Sueishi, K.

In: Transplantation Proceedings, Vol. 41, No. 5, 01.06.2009, p. 1504-1507.

Research output: Contribution to journalArticle

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