Examination of the usefulness of next-generation sequencing in mixed DNA samples

Fumi Momota, Akiko Tsuji, Atsushi Ishiko, Noriaki Ikeda

Research output: Contribution to journalArticlepeer-review

Abstract

The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.

Original languageEnglish
Article number101874
JournalLegal Medicine
Volume51
DOIs
Publication statusPublished - Jul 2021

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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