Excision of one of two defective P elements as the cause of alternate mutational events (sn+ and sne) of the singed bristle allele snw in Drosophila melanogaster

Eiji Nitasaka, Tsuneyuki Yamazaki

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7 Citations (Scopus)

Abstract

The X-linked singed locus is concerned with the bristle phenotype and female sterility, and is known as a hot spot of P element insertion. A moderate allele of singed, singed-weak (snw) (Engels, 1979; 1984) is inserted with P elements. It is used as an index of P element activity, since it mutates at a high frequency to either a more extreme allele, singed-extreme (sne), or to a phenotype that is equivalent to the wild type (SK+) when an autonomous P element exists. We show here that mw is inserted with two defective P elements in reverse orientation, and the two alternate mutational events (sn+ and sne) are caused by the excision of one or the other of the P elements present in the singed gene. It is interesting that sn+ and sne are inserted with a single P element in the same position, but show very different phenotypes. The insertional sites of P elements in the singed locus possibly contain an unidentified repetitive sequence, which is repeated dozens of times per haploid genome of the wild-type strain Canton-S.

Original languageEnglish
Pages (from-to)303-312
Number of pages10
JournalThe Japanese Journal of Genetics
Volume63
Issue number4
DOIs
Publication statusPublished - Mar 28 1988

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Drosophila melanogaster
Alleles
Phenotype
Female Infertility
Nucleic Acid Repetitive Sequences
Haploidy
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "Excision of one of two defective P elements as the cause of alternate mutational events (sn+ and sne) of the singed bristle allele snw in Drosophila melanogaster",
abstract = "The X-linked singed locus is concerned with the bristle phenotype and female sterility, and is known as a hot spot of P element insertion. A moderate allele of singed, singed-weak (snw) (Engels, 1979; 1984) is inserted with P elements. It is used as an index of P element activity, since it mutates at a high frequency to either a more extreme allele, singed-extreme (sne), or to a phenotype that is equivalent to the wild type (SK+) when an autonomous P element exists. We show here that mw is inserted with two defective P elements in reverse orientation, and the two alternate mutational events (sn+ and sne) are caused by the excision of one or the other of the P elements present in the singed gene. It is interesting that sn+ and sne are inserted with a single P element in the same position, but show very different phenotypes. The insertional sites of P elements in the singed locus possibly contain an unidentified repetitive sequence, which is repeated dozens of times per haploid genome of the wild-type strain Canton-S.",
author = "Eiji Nitasaka and Tsuneyuki Yamazaki",
year = "1988",
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T1 - Excision of one of two defective P elements as the cause of alternate mutational events (sn+ and sne) of the singed bristle allele snw in Drosophila melanogaster

AU - Nitasaka, Eiji

AU - Yamazaki, Tsuneyuki

PY - 1988/3/28

Y1 - 1988/3/28

N2 - The X-linked singed locus is concerned with the bristle phenotype and female sterility, and is known as a hot spot of P element insertion. A moderate allele of singed, singed-weak (snw) (Engels, 1979; 1984) is inserted with P elements. It is used as an index of P element activity, since it mutates at a high frequency to either a more extreme allele, singed-extreme (sne), or to a phenotype that is equivalent to the wild type (SK+) when an autonomous P element exists. We show here that mw is inserted with two defective P elements in reverse orientation, and the two alternate mutational events (sn+ and sne) are caused by the excision of one or the other of the P elements present in the singed gene. It is interesting that sn+ and sne are inserted with a single P element in the same position, but show very different phenotypes. The insertional sites of P elements in the singed locus possibly contain an unidentified repetitive sequence, which is repeated dozens of times per haploid genome of the wild-type strain Canton-S.

AB - The X-linked singed locus is concerned with the bristle phenotype and female sterility, and is known as a hot spot of P element insertion. A moderate allele of singed, singed-weak (snw) (Engels, 1979; 1984) is inserted with P elements. It is used as an index of P element activity, since it mutates at a high frequency to either a more extreme allele, singed-extreme (sne), or to a phenotype that is equivalent to the wild type (SK+) when an autonomous P element exists. We show here that mw is inserted with two defective P elements in reverse orientation, and the two alternate mutational events (sn+ and sne) are caused by the excision of one or the other of the P elements present in the singed gene. It is interesting that sn+ and sne are inserted with a single P element in the same position, but show very different phenotypes. The insertional sites of P elements in the singed locus possibly contain an unidentified repetitive sequence, which is repeated dozens of times per haploid genome of the wild-type strain Canton-S.

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