Excitatory amino acid response in isolated nucleus tractus solitarii neurons of the rat

Takashi Nakagawa, Tetsuya Shirasaki, Minoru Wakamori, Atsuo Fukuda, Norio Akaike

Research output: Contribution to journalArticle

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Abstract

The excitatory amino-acid-induced currents in nucleus tractus solitarii neurons freshly isolated from rats were investigated in a whole-cell recording mode using a conventional patch-clamp technique. At a holding potential of -70 mV, l-glutamate (Glu), N-methyl-d-aspartate (NMDA) with 10-9 M glycine, kainate (KA), quisqualate (QA) and l-aspartate (Asp) evoked inward currents. The currents increased in a sigmoidal fashion with increasing agonists concentration. The half-maximum concentration (EC50) values were 5×10-5 M for Glu, 10-6 M for QA, 10-4 M for KA, 6×10-5 M for NMDA and 5×10-5 M for Asp. The Hill coefficients of the Glu-, QA-, KA-, NMDA- and Asp-induced respones were 1.0, 1.3, 1.1, 1.3 and 1.1, respectively. The Glu-, QA-, NMDA- and Asp-induced currents consisted of a transient initial peak and a successive steady-state component showing no desensitization. These currents had the same reversal potential near +5 mV. In the current-voltage (I-V) relationships for the Glu-, NMDA- and Asp-induced currents, slight outward rectifications were observed in Mg2+-free external solution at membrane potentials negative to 0 mV. In the presence of extracellular Mg2+, the currents induced by Glu, NMDA and Asp were suppressed at negative membrane potentials, but the suppression was less for the Glu response. The I-V relationships for QA- and KA-induced responses were almost linear at a membrane potential between -90 and +50 mV with or without the presence of Mg2+.

Original languageEnglish
Pages (from-to)114-123
Number of pages10
JournalNeuroscience Research
Volume8
Issue number2
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Excitatory Amino Acids
Solitary Nucleus
Aspartic Acid
Neurons
Quisqualic Acid
Kainic Acid
Membrane Potentials
Patch-Clamp Techniques
Glycine
Glutamic Acid

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Excitatory amino acid response in isolated nucleus tractus solitarii neurons of the rat. / Nakagawa, Takashi; Shirasaki, Tetsuya; Wakamori, Minoru; Fukuda, Atsuo; Akaike, Norio.

In: Neuroscience Research, Vol. 8, No. 2, 01.01.1990, p. 114-123.

Research output: Contribution to journalArticle

Nakagawa, Takashi ; Shirasaki, Tetsuya ; Wakamori, Minoru ; Fukuda, Atsuo ; Akaike, Norio. / Excitatory amino acid response in isolated nucleus tractus solitarii neurons of the rat. In: Neuroscience Research. 1990 ; Vol. 8, No. 2. pp. 114-123.
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