Excitatory effects of ATP on rat dorsomedial hypothalamic neurons

N. Matsumoto, M. Sorimachi, N. Akaike

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca2+ ([Ca]i). The rise in [Ca]i was dependent on external Ca2+ and Na+, was blocked by Ca2+ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca 2+ channels, resulting in an increase in [Ca]i.

Original languageEnglish
Pages (from-to)234-237
Number of pages4
JournalBrain Research
Volume1009
Issue number1-2
DOIs
Publication statusPublished - May 29 2004

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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