Existence of muscarinic suppression of a K current in PC-12 pheochromocytoma cells

K. Nakazawa, K. Fujimori, A. Takanaka, Kazuhide Inoue

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Muscarinic influence on membrane currents of PC-12 pheochromocytoma cells were investigated with whole cell voltage-clamp methods. An outward K current was observed when depolarizing voltage steps were applied to the cells. Methacholine (MCh, 300 μM), a selective agonist for muscarinic receptors, partially suppressed the K current, and the suppression was enhanced by removal of external Ca. The effect of MCh was antagonized by a low dose (100 nM) of atropine. Nicotine (10 μM) induced an inward current in these cells but did not affect the K current activated by depolarizing voltage steps. A Ba current flowing through voltage-gated Ca channels was not changed by MCh. The results indicate the existence of a MCh-sensitive K current in PC-12 cells and suggest that the membrane currents of these cells are modulated by cholinergic agents through muscarinic mechanisms in addition to well-known nicotinic mechanisms.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume257
Issue number5
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Pheochromocytoma
Cholinergic Agents
Electric potential
Membranes
Methacholine Chloride
Clamping devices
Muscarinic Receptors
Nicotine
Atropine
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Existence of muscarinic suppression of a K current in PC-12 pheochromocytoma cells. / Nakazawa, K.; Fujimori, K.; Takanaka, A.; Inoue, Kazuhide.

In: American Journal of Physiology - Cell Physiology, Vol. 257, No. 5, 1989.

Research output: Contribution to journalArticle

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