Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpoB)

Hitoshi Inada, Motoaki Seki, Hiromichi Morikawa, Mitsuo Nishimura, Koh Iba

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The plastid gene rpoB encodes a plastid-specific, DNA-dependent RNA polymerase. A study of a barley mutant, albostrians, indicated that rpoB itself is transcribed by a nuclear-encoded RNA polymerase. However, the molecular nature of the nuclear-encoded RNA polymerase and mechanisms of the transcriptional regulation of rpoB expression have not been elucidated. Using an in vivo transient assay system for gene expression in plastids, the 5'-flanking region of rpoB from Arabidopsis thaliana in plastids of cultured BY-2 tobacco cells was analyzed. A series of deletion analyses of the 5'-flanking region indicated that there were two positive and one negative regulatory regions for rpoB expression. Comparison of the 5'-flanking sequences of rpoB among several plant species revealed the existence of three conserved motifs in these regions.

Original languageEnglish
Pages (from-to)883-890
Number of pages8
JournalPlant Journal
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1997

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

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