Exogenous gene transfection into quail embryo using cationic lipid vesicles

Satoshi Oguchi, Masamichi Kamihira, Jun You, Ayuko Tachibana, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We have previously reported a simple procedure for gene transfection mediated by cationic lipid vesicles for animal cells, in which a commercially available synthetic cationic surfactant, dimethyidioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. The transfection method was associated with low cytotoxicity and high transfection efficiency. In the present study, the method was applied for gene transfection into quail embryos. The complex solution of lipid vesicles and pmiwZ plasmid, a β-galactosidase expression vector under the control of β-actin promoter as a reporter, was injected into quail embryos at various developmental stages using a glass micropipette. After incubation at 37.7 °C for 3-4 d, the embryos were fixed and stained with X-gal solution. Under optimal conditions, about 80% of quail embryos expressed β-galactosidase in limited regions of tissues and organs with high viability. Moreover, 35% of the treated embryos (15/43) hatched following in vitro embryo culture, using the method developed by us.

Original languageEnglish
Pages (from-to)118-120
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume86
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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