Exogenously hypercholesterolemic rats, compared with their progenitor Sprague-Dawley rats, have altered mRNAs for cholesterol 7α-hydroxylase and low-density-lipoprotein receptor and activities of cholesterol 7α- hydroxylase and acyl-CoA: Cholesterol acyltransferase in the liver in response to dietary cholesterol

Koji Nagao, Masanobu Sakono, Hideki Sakurai, Masao Sato, Katsumi Imaizumi

Research output: Contribution to journalArticle

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Abstract

Exogenously hypercholesterolemic (ExHC) rats promptly increase serum cholesterol concentration in response to dietary cholesterol. To examine underlying mechanism(s) for this susceptibility, responses of mRNAs for cholesterol metabolism-related proteins and their activities in the liver to dietary cholesterol were compared between ExHC rats and their progenitor Sprague-Dawley rats. ExHC rats slightly decreased the abundance of low- density-lipoprotein (LDL) receptor mRNA in response to dietary cholesterol, although the amount of LDL receptor was not influenced. The abundance of cholesterol 7α-hydroxylase mRNA and the enzyme activity in response to dietary cholesterol were greater in ExHC rats, but the fecal excretion of bile acid was comparable between the strain. Dietary cholesterol-dependent elevation of acyl-CoA:cholesterol acyltransferase activity was greater in ExHC rats. The concentration of liver triacylglycerols was markedly lower in ExHC rats. These results suggest that ExHC rats may increase serum cholesterol by increasing hepatic secretion of cholesterol ester-rich particles.

Original languageEnglish
Pages (from-to)949-955
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume118
Issue number4
DOIs
Publication statusPublished - Jan 1 1997

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Cholesterol 7-alpha-Hydroxylase
Sterol O-Acyltransferase
Dietary Cholesterol
LDL Receptors
Liver
Sprague Dawley Rats
Rats
Messenger RNA
Cholesterol
Cholesterol Esters
Bile Acids and Salts
Serum
Enzyme activity
Metabolism
Triglycerides
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology

Cite this

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title = "Exogenously hypercholesterolemic rats, compared with their progenitor Sprague-Dawley rats, have altered mRNAs for cholesterol 7α-hydroxylase and low-density-lipoprotein receptor and activities of cholesterol 7α- hydroxylase and acyl-CoA: Cholesterol acyltransferase in the liver in response to dietary cholesterol",
abstract = "Exogenously hypercholesterolemic (ExHC) rats promptly increase serum cholesterol concentration in response to dietary cholesterol. To examine underlying mechanism(s) for this susceptibility, responses of mRNAs for cholesterol metabolism-related proteins and their activities in the liver to dietary cholesterol were compared between ExHC rats and their progenitor Sprague-Dawley rats. ExHC rats slightly decreased the abundance of low- density-lipoprotein (LDL) receptor mRNA in response to dietary cholesterol, although the amount of LDL receptor was not influenced. The abundance of cholesterol 7α-hydroxylase mRNA and the enzyme activity in response to dietary cholesterol were greater in ExHC rats, but the fecal excretion of bile acid was comparable between the strain. Dietary cholesterol-dependent elevation of acyl-CoA:cholesterol acyltransferase activity was greater in ExHC rats. The concentration of liver triacylglycerols was markedly lower in ExHC rats. These results suggest that ExHC rats may increase serum cholesterol by increasing hepatic secretion of cholesterol ester-rich particles.",
author = "Koji Nagao and Masanobu Sakono and Hideki Sakurai and Masao Sato and Katsumi Imaizumi",
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T1 - Exogenously hypercholesterolemic rats, compared with their progenitor Sprague-Dawley rats, have altered mRNAs for cholesterol 7α-hydroxylase and low-density-lipoprotein receptor and activities of cholesterol 7α- hydroxylase and acyl-CoA

T2 - Cholesterol acyltransferase in the liver in response to dietary cholesterol

AU - Nagao, Koji

AU - Sakono, Masanobu

AU - Sakurai, Hideki

AU - Sato, Masao

AU - Imaizumi, Katsumi

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Exogenously hypercholesterolemic (ExHC) rats promptly increase serum cholesterol concentration in response to dietary cholesterol. To examine underlying mechanism(s) for this susceptibility, responses of mRNAs for cholesterol metabolism-related proteins and their activities in the liver to dietary cholesterol were compared between ExHC rats and their progenitor Sprague-Dawley rats. ExHC rats slightly decreased the abundance of low- density-lipoprotein (LDL) receptor mRNA in response to dietary cholesterol, although the amount of LDL receptor was not influenced. The abundance of cholesterol 7α-hydroxylase mRNA and the enzyme activity in response to dietary cholesterol were greater in ExHC rats, but the fecal excretion of bile acid was comparable between the strain. Dietary cholesterol-dependent elevation of acyl-CoA:cholesterol acyltransferase activity was greater in ExHC rats. The concentration of liver triacylglycerols was markedly lower in ExHC rats. These results suggest that ExHC rats may increase serum cholesterol by increasing hepatic secretion of cholesterol ester-rich particles.

AB - Exogenously hypercholesterolemic (ExHC) rats promptly increase serum cholesterol concentration in response to dietary cholesterol. To examine underlying mechanism(s) for this susceptibility, responses of mRNAs for cholesterol metabolism-related proteins and their activities in the liver to dietary cholesterol were compared between ExHC rats and their progenitor Sprague-Dawley rats. ExHC rats slightly decreased the abundance of low- density-lipoprotein (LDL) receptor mRNA in response to dietary cholesterol, although the amount of LDL receptor was not influenced. The abundance of cholesterol 7α-hydroxylase mRNA and the enzyme activity in response to dietary cholesterol were greater in ExHC rats, but the fecal excretion of bile acid was comparable between the strain. Dietary cholesterol-dependent elevation of acyl-CoA:cholesterol acyltransferase activity was greater in ExHC rats. The concentration of liver triacylglycerols was markedly lower in ExHC rats. These results suggest that ExHC rats may increase serum cholesterol by increasing hepatic secretion of cholesterol ester-rich particles.

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