Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA

Takuya Yoda, Maiko Tanabe, Toshiyuki Tsuji, Takao Yoda, Sonoko Ishino, Tsuyoshi Shirai, Yoshizumi Ishino, Haruko Takeyama, Hirokazu Nishida

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Abstract

Family B DNA polymerases comprise polymerase and 3′ ->5′ exonuclease domains, and detect a mismatch in a newly synthesized strand to remove it in cooperation with Proliferating cell nuclear antigen (PCNA), which encircles the DNA to provide a molecular platform for efficient protein-protein and protein-DNA interactions during DNA replication and repair. Once the repair is completed, the enzyme must stop the exonucleolytic process and switch to the polymerase mode. However, the cue to stop the degradation is unclear. We constructed several PCNA mutants and found that the exonuclease reaction was enhanced in the mutants lacking the conserved basic patch, located on the inside surface of PCNA. These mutants may mimic the Pol/PCNA complex processing the mismatched DNA, in which PCNA cannot interact rigidly with the irregularly distributed phosphate groups outside the dsDNA. Indeed, the exonuclease reaction with the wild type PCNA was facilitated by mismatched DNA substrates. PCNA may suppress the exonuclease reaction after the removal of the mismatched nucleotide. PCNA seems to act as a "brake" that stops the exonuclease mode of the DNA polymerase after the removal of a mismatched nucleotide from the substrate DNA, for the prompt switch to the DNA polymerase mode.

Original languageEnglish
Article number44582
JournalScientific reports
Volume7
DOIs
Publication statusPublished - Mar 16 2017

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Archaeal DNA
Exonucleases
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
DNA
Nucleotides
Phosphodiesterase I
Proteins
Antigen Presentation
DNA Replication
DNA Repair
Cues
Phosphates

All Science Journal Classification (ASJC) codes

  • General

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Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA. / Yoda, Takuya; Tanabe, Maiko; Tsuji, Toshiyuki; Yoda, Takao; Ishino, Sonoko; Shirai, Tsuyoshi; Ishino, Yoshizumi; Takeyama, Haruko; Nishida, Hirokazu.

In: Scientific reports, Vol. 7, 44582, 16.03.2017.

Research output: Contribution to journalArticle

Yoda, Takuya ; Tanabe, Maiko ; Tsuji, Toshiyuki ; Yoda, Takao ; Ishino, Sonoko ; Shirai, Tsuyoshi ; Ishino, Yoshizumi ; Takeyama, Haruko ; Nishida, Hirokazu. / Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA. In: Scientific reports. 2017 ; Vol. 7.
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AU - Yoda, Takao

AU - Ishino, Sonoko

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