TY - JOUR
T1 - Exosomal miR-155 Derived from Hepatocellular Carcinoma Cells Under Hypoxia Promotes Angiogenesis in Endothelial Cells
AU - Matsuura, Yusuke
AU - Wada, Hiroshi
AU - Eguchi, Hidetoshi
AU - Gotoh, Kunihito
AU - Kobayashi, Shogo
AU - Kinoshita, Mitsuru
AU - Kubo, Masahiko
AU - Hayashi, Koji
AU - Iwagami, Yoshifumi
AU - Yamada, Daisaku
AU - Asaoka, Tadafumi
AU - Noda, Takehiro
AU - Kawamoto, Koichi
AU - Takeda, Yutaka
AU - Tanemura, Masahiro
AU - Umeshita, Koji
AU - Doki, Yuichiro
AU - Mori, Masaki
N1 - Funding Information:
Acknowledgments This work was supported by JSPS KAKENHI
Funding Information:
This work was supported by JSPS KAKENHI Grant No. JP 26462043.
Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - Purpose: In this study, we aim to clarify whether exosomes secreted from hepatocellular carcinoma (HCC) cells under hypoxia affect angiogenesis in endothelial cells. Methods: Exosomes derived from human liver cancer cell lines were cultured under hypoxic or normoxic conditions for 24 h, isolated using ExoQuick-TC ® , and co-cultured with HUVECs to evaluate angiogenic activity. We also evaluated the expression of miR-155 in the exosomes from 40 patients with HCC. Results: Exosomes under hypoxia remarkably enhanced tube formation of HUVECs. Both cellular and exosomal miR-155 were significantly up-regulated under hypoxic conditions. Knockdown of miR-155 in HCC cells attenuated the promotion of tube formation by exosomes under hypoxia in HUVECs, and high expression of exosomal miR-155 in preoperative plasma was significantly correlated with early recurrence. Conclusion: These results suggest that exosomes derived from HCC cells under hypoxia induce tube formation of HUVECs and that exosomal miR-155 may affect angiogenic activity in HCC.
AB - Purpose: In this study, we aim to clarify whether exosomes secreted from hepatocellular carcinoma (HCC) cells under hypoxia affect angiogenesis in endothelial cells. Methods: Exosomes derived from human liver cancer cell lines were cultured under hypoxic or normoxic conditions for 24 h, isolated using ExoQuick-TC ® , and co-cultured with HUVECs to evaluate angiogenic activity. We also evaluated the expression of miR-155 in the exosomes from 40 patients with HCC. Results: Exosomes under hypoxia remarkably enhanced tube formation of HUVECs. Both cellular and exosomal miR-155 were significantly up-regulated under hypoxic conditions. Knockdown of miR-155 in HCC cells attenuated the promotion of tube formation by exosomes under hypoxia in HUVECs, and high expression of exosomal miR-155 in preoperative plasma was significantly correlated with early recurrence. Conclusion: These results suggest that exosomes derived from HCC cells under hypoxia induce tube formation of HUVECs and that exosomal miR-155 may affect angiogenic activity in HCC.
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U2 - 10.1007/s10620-018-5380-1
DO - 10.1007/s10620-018-5380-1
M3 - Article
C2 - 30465177
AN - SCOPUS:85056814068
SN - 0163-2116
VL - 64
SP - 792
EP - 802
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 3
ER -
