Exosomes secreted from monocyte-derived dendritic cells support in vitro naive CD4+ T cell survival through NF-κB activation

Kotaro Matsumoto, Takashi Morisaki, Hideo Kuroki, Makoto Kubo, Hideya Onishi, Katsuya Nakamura, Chihiro Nakahara, Hirotaka Kuga, Eishi Baba, Masafumi Nakamura, Kazuho Hirata, Masao Tanaka, Mitsuo Katano

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We investigated the effect of exosomes secreted from human monocyte-derived dendritic cells (Mo-DCs), which are generated from PBMCs in response to treatment with GM-CSF and IL-4, on naive CD4+ T cell survival in vitro. Exosomes isolated from culture supernatants of Mo-DCs (>90% purity) were purified with anti-HLA-DP, -DQ, -DR-coated paramagnetic beads. Purified exosomes prolonged the survival of naive CD4+ T cells (>98% purity) in vitro. Treatment with neutralizing mAb against HLA-DR significantly decreased the supportive effect of purified exosomes on CD4+ T cell survival. Exosomes increased nuclear translocation of NF-κB in naive CD4+ T cells, and NF-κB activation was significantly suppressed by anti-HLA-DR mAb or NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC). In addition, PDTC inhibited the effect of exosomes on naive CD4+ T cell survival. Thus, exosomes secreted by Mo-DCs appear to support naive CD4+ T cell survival via NF-κB activation induced by interaction of HLA-DR and TCRs.

Original languageEnglish
Pages (from-to)20-29
Number of pages10
JournalCellular Immunology
Volume231
Issue number1-2
DOIs
Publication statusPublished - Sep 2004

All Science Journal Classification (ASJC) codes

  • Immunology

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