Expansion of genes encoding complement components in bony fish

Biological implications of the complement diversity

Miki Nakao, Junichi Mutsuro, Makiko Nakahara, Yoko Kato, Tomoki Yano

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The complement system is a major humoral component of vertebrate defenses for tagging and killing target microorganisms. Recent molecular analyses have uncovered a striking feature of bony fish complement, namely that several complement components are encoded by multiple genes. In this review, the structural diversity of C3, C4, C5, factor B, C2, C1r/s and MASP are discussed with special reference to their functional differentiation, mainly focusing on the common carp (Cyprinus carpio), a tetraploidized teleost. In carp, all the members (C3, C4, C5 and a non-complement protein α2-macroglobulin) of the thioester-containing protein family are present in multiple isotypes, differing in the primary structures of various functional sites. Three factor B/C2-like isotypes identified in carp showed distinct expression pattern (sites and inducibility), with one behaving as an acute-phase reactant. Two C1r/C1s/MASP2-like isotypes also contain an amino acid substitution that likely affects their substrate specificity. Overall, the present data suggest that the expanded genes of the carp complement system produce more diversified functional components than are known for mammals. The biological significance of this diversity is discussed.

Original languageEnglish
Pages (from-to)749-762
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume27
Issue number9
DOIs
Publication statusPublished - Jan 1 2003

Fingerprint

Carps
Fishes
Complement Factor B
Genes
Mannose-Binding Protein-Associated Serine Proteases
Macroglobulins
Acute-Phase Proteins
Biodiversity
Amino Acid Substitution
Substrate Specificity
Vertebrates
Mammals
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Expansion of genes encoding complement components in bony fish : Biological implications of the complement diversity. / Nakao, Miki; Mutsuro, Junichi; Nakahara, Makiko; Kato, Yoko; Yano, Tomoki.

In: Developmental and Comparative Immunology, Vol. 27, No. 9, 01.01.2003, p. 749-762.

Research output: Contribution to journalArticle

Nakao, Miki ; Mutsuro, Junichi ; Nakahara, Makiko ; Kato, Yoko ; Yano, Tomoki. / Expansion of genes encoding complement components in bony fish : Biological implications of the complement diversity. In: Developmental and Comparative Immunology. 2003 ; Vol. 27, No. 9. pp. 749-762.
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