Experimental allergic encephalomyelitis in rabbits. A major encephalitogenic determinant withinresidues 1-44 of myelin basic protein

Jun ichi Kira, Martha L. Bacon, Russell E. Martenson, Gladys E. Deibler, Marian W. Kies, Ellsworth C. Alvord

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Experimental allergic encephalomyelitis could be induced in rabbits by injection in Freund's complete adjuvant of either peptide 1-44 or peptide 45-87 of rabbit myelin basic protein. In order to localize the encephalitogenic determinant present in peptide 1-44, several smaller derivative peptides were prepared and examined. Peptic peptide 15-44 and thrombic peptide 1-31 were as active as peptide 1-44, whereas peptic peptides 1-14 and 18-38 and BrCN peptide 22-44 were virtually inactive. Weak activity was shown by BrCN peptide 1-21. These results provide evidence that a major encephalitogenic determinant present in peptide 1-44 lies within sequence 15-31. The encephalitogenic activity of peptide 15-44 was essentially destroyed by oxidation of methionine-21 to methionine sulfoxide; methylation of Met-21, on the other hand, appeared to be relatively ineffective in eliminating the encephalitogenicity of peptide 1-44.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalJournal of Neuroimmunology
Volume12
Issue number3
DOIs
Publication statusPublished - Sep 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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