Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori

Kohji Yamamoto; Hirofumi Ichinose; Yoichi Aso; Hiroshi Fujii

doi:10.1016/j.pestbp.2009.11.006

Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori

Kohji Yamamoto, Hirofumi Ichinose, Yoichi Aso, Hiroshi Fujii

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Pesticide Biochemistry and Physiology
Volume	97
Issue number	1
DOIs	https://doi.org/10.1016/j.pestbp.2009.11.006
Publication status	Published - May 1 2010

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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title = "Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori",

abstract = "Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.",

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AU - Yamamoto, Kohji

AU - Ichinose, Hirofumi

AU - Aso, Yoichi

AU - Fujii, Hiroshi

PY - 2010/5/1

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