Abstract
Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.
| Original language | English |
|---|---|
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| Journal | Pesticide Biochemistry and Physiology |
| Volume | 97 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 1 2010 |
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All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis
Cite this
Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori. / Yamamoto, Kohji; Ichinose, Hirofumi; Aso, Yoichi; Fujii, Hiroshi.
In: Pesticide Biochemistry and Physiology, Vol. 97, No. 1, 01.05.2010, p. 1-6.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori
AU - Yamamoto, Kohji
AU - Ichinose, Hirofumi
AU - Aso, Yoichi
AU - Fujii, Hiroshi
PY - 2010/5/1
Y1 - 2010/5/1
N2 - Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.
AB - Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.
UR - http://www.scopus.com/inward/record.url?scp=77950595059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950595059&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2009.11.006
DO - 10.1016/j.pestbp.2009.11.006
M3 - Article
AN - SCOPUS:77950595059
VL - 97
SP - 1
EP - 6
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
SN - 0048-3575
IS - 1
ER -