Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

Eiki Takahashi, Norimasa Miyamoto, Noriko Kajiwara, Keiko Furuya, Keiko Yanai-Taniguchi, Fumihiro Sugiyama, Ken Ichi Yagami

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of β-galactosidase activity. Themes: Cellular and molecular biology. Topics: Staining, tracing, and imaging techniques. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalBrain Research Protocols
Volume5
Issue number2
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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