Expression analysis of the spi gene in the pock-forming plasmid pSA1.1 from Streptomyces azureus and localization of its product during differentiation

Katsumi Doi, Yukiko Ohyama, Eiji Yokoyama, Takashi Nishiyama, Yasuhiro Fujino, Yuko Nagayoshi, Toshihisa Ohshima, Seiya Ogata

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2 Citations (Scopus)

Abstract

The sporulation inhibitory gene spi in the pockforming conjugative plasmid pSA1.1 of Streptomyces azureus was introduced into cells via a high or low copy number vector to examine the effect of gene dosage on the growth of Streptomyces lividans TK24 as a host. In transformants carrying a high spi copy number, nutrient mycelial growth was inhibited, as was morphological differentiation from substrate mycelium to aerial mycelium on solid media. The degree of inhibition depended on the spi gene dosage, but the presence of pSA1.1 imp genes, which encode negative repressor proteins for spi, relieved the inhibition. Confocal images of Spi tagged with enhanced green fluorescent protein in cells on solid media revealed that spi expression was initiated at the time of elongation of substrate mycelium, that its expression increased dramatically at septation in aerial hyphae, and that the expression was maximal during prespore formation. Expression of spi covered the whole of the hyphae, and the level of expression at the tip of the hyphae during prespore formation was about sixfold greater than during substrate mycelial growth and threefold greater than during aerial mycelial growth. Thus, localized expression of spi at particular times may inhibit sporulation until triggering imp expression to repress its inhibitory effects.

Original languageEnglish
Pages (from-to)707-716
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume95
Issue number3
DOIs
Publication statusPublished - Aug 1 2012

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Streptomyces
Hyphae
Mycelium
Plasmids
Gene Dosage
Growth
Genes
Streptomyces lividans
Repressor Proteins
Food

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Expression analysis of the spi gene in the pock-forming plasmid pSA1.1 from Streptomyces azureus and localization of its product during differentiation. / Doi, Katsumi; Ohyama, Yukiko; Yokoyama, Eiji; Nishiyama, Takashi; Fujino, Yasuhiro; Nagayoshi, Yuko; Ohshima, Toshihisa; Ogata, Seiya.

In: Applied Microbiology and Biotechnology, Vol. 95, No. 3, 01.08.2012, p. 707-716.

Research output: Contribution to journalArticle

Doi, Katsumi ; Ohyama, Yukiko ; Yokoyama, Eiji ; Nishiyama, Takashi ; Fujino, Yasuhiro ; Nagayoshi, Yuko ; Ohshima, Toshihisa ; Ogata, Seiya. / Expression analysis of the spi gene in the pock-forming plasmid pSA1.1 from Streptomyces azureus and localization of its product during differentiation. In: Applied Microbiology and Biotechnology. 2012 ; Vol. 95, No. 3. pp. 707-716.
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