Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells

H. Kanouchi, T. Oka, K. Asagi, Hirofumi Tachibana, K. Yamada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To clarify the biological role of kidney perchloric acid-soluble protein 1 (K-PSP1), its expression and intracellular distribution were examined in normal rat kidney epithelial NRK-52E cells. K-PSP1 expression was low during the proliferating phase and high in the stationary phase, and shown to have a negative relationship with the protein-synthesizing activity of the cells. Immunocytochemical studies revealed that K-PSP1 is predominantly located in the cytosol, especially in endoplasmic reticulum and Golgi apparatus of proliferating cells. In the stationary phase, K-PSP1 was not detected immunologically even though protein and mRNA expression were high. This disappearance of reactivity with anti-serum seems to be due to a conformational change in K-PSP1 induced by unknown factors. These results suggest that the role of K-PSP1 is to regulate cell proliferation, and this may be related to a previously reported ability to inhibit protein synthesis.

Original languageEnglish
Pages (from-to)1103-1108
Number of pages6
JournalCellular and Molecular Life Sciences
Volume57
Issue number7
DOIs
Publication statusPublished - Jan 1 2000

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Kidney
Proteins
Perchloric Acid
Cell proliferation
Golgi Apparatus
Endoplasmic Reticulum
Cytosol
Rats
Cells
Cell Proliferation
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells. / Kanouchi, H.; Oka, T.; Asagi, K.; Tachibana, Hirofumi; Yamada, K.

In: Cellular and Molecular Life Sciences, Vol. 57, No. 7, 01.01.2000, p. 1103-1108.

Research output: Contribution to journalArticle

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