Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines

Takeshi Yoshida, Hiroto Izumi, Takeshi Uchiumi, Yasuyuki Sasaguri, Akihide Tanimoto, Tetsuro Matsumoto, Seiji Naito, Kimitoshi Kohno

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Abstract

The transcriptional regulation of the germ cell-specific cold-shock domain protein dbpC/Contrin was investigated, and the promoter region between -272 and -253 relative to the transcription start site was shown to be critical for the manifestation of cell-type specific transcription. In vivo footprint analysis demonstrated that the E-box located between -272 and -253 is protected in the dbpC/Contrin-positive germ cell tumor cell lines NEC8 and TERA1, but not in the dbpC/Contrin-negative bladder cancer cell line T24 or ovarian cancer cell line A2780. The promoter activity of the dbpC/Contrin gene was transactivated by co-transfection with c-Myc and the N-Myc expression plasmid. Western blotting analysis clearly showed that N-Myc is highly expressed in both NEC8 and TERA1 cells, and that c-Myc is expressed in both T24 and A2780 cells. These data demonstrate that cell-type specific dbpC/Contrin expression in germ cells is regulated by N-Myc. In addition, dbpC/Contrin is localized mainly in the cytoplasm of NEC8 and TERA1 cells, but is translocated to the nucleus when its C-terminal region is partially deleted. Our findings also suggest that dbpC/Contrin can be used as a molecular tool for the detection of germ cell tumors.

Original languageEnglish
Pages (from-to)80-88
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1759
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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