Expression and characterization of myristoylated preS1-conjugated nanocages for targeted cell delivery

Masaharu Murata, Jing Shu Piao, Sayoko Narahara, Takahito Kawano, Nobuhito Hamano, Jeong Hun Kang, Daisuke Asai, Ryo Ugawa, Makoto Hashizume

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lipid modification of proteins plays key roles in cellular signaling pathways. We describe the development of myristoylated preS1-nanocages (myr-preS1-nanocages) that specifically target human hepatocyte-like HepaRG cells in which a specific receptor-binding peptide (preS1) is joined to the surface of naturally occurring ferritin cages. Using a genetic engineering approach, the preS1 peptide was joined to the N-terminal regions of the ferritin cage via flexible linker moieties. Myristoylation of the preS1 peptide was achieved by co-expression with yeast N-myristoyltransferase-1 in the presence of myristic acid in Escherichia coli cells. The myristoylated preS1-nanocages exhibited significantly greater specificity for human hepatocyte-like HepaRG cells than the unmyristoylated preS1-nanocages. These results suggest that the lipid-modified nanocages have great potential for effective targeted delivery to specific cells.

Original languageEnglish
Pages (from-to)52-56
Number of pages5
JournalProtein Expression and Purification
Volume110
DOIs
Publication statusPublished - Jan 1 2015

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Ferritins
Hepatocytes
Lipids
Peptides
Genetic Engineering
Peptide Receptors
Myristic Acid
Yeasts
Escherichia coli
Proteins
glycylpeptide N-tetradecanoyltransferase

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Expression and characterization of myristoylated preS1-conjugated nanocages for targeted cell delivery. / Murata, Masaharu; Piao, Jing Shu; Narahara, Sayoko; Kawano, Takahito; Hamano, Nobuhito; Kang, Jeong Hun; Asai, Daisuke; Ugawa, Ryo; Hashizume, Makoto.

In: Protein Expression and Purification, Vol. 110, 01.01.2015, p. 52-56.

Research output: Contribution to journalArticle

Murata, M, Piao, JS, Narahara, S, Kawano, T, Hamano, N, Kang, JH, Asai, D, Ugawa, R & Hashizume, M 2015, 'Expression and characterization of myristoylated preS1-conjugated nanocages for targeted cell delivery', Protein Expression and Purification, vol. 110, pp. 52-56. https://doi.org/10.1016/j.pep.2014.12.001
Murata, Masaharu ; Piao, Jing Shu ; Narahara, Sayoko ; Kawano, Takahito ; Hamano, Nobuhito ; Kang, Jeong Hun ; Asai, Daisuke ; Ugawa, Ryo ; Hashizume, Makoto. / Expression and characterization of myristoylated preS1-conjugated nanocages for targeted cell delivery. In: Protein Expression and Purification. 2015 ; Vol. 110. pp. 52-56.
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