Abstract
Expression of bradykinin (BK) receptors and their cellular function were investigated in microglia. Microglial cells were isolated from mixed cultures of cerebrocortical cells from postnatal day 3 Wistar rats. Reverse transcription-PCR (RT-PCR) showed that rat primary microglia express mRNAs for the type 2 bradykinin (B2) receptor subtype but not the type 1 (B1) receptor subtype under our experimental condition. However, the expression of B1 receptor was greatly up-regulated after the treatment of microglia with BK for 24 hours. The expression of B2 receptor in microglia was further confirmed by immunocytochemistry. Membrane currents were measured using whole-cell recording under voltage-clamp conditions. In 14% of patched cells (12/85 cells), BK (100-200 nM) induced an outward current at the holding potential of -20 mV, with oscillations in 2 cases. The BK-induced outward current was transient and desensitized rapidly. TEA inhibited the BK-induced outward current in a dose-dependent manner. These results suggest that microglia express B2 receptors and presumably increase the intracellular Ca2+ concentration via inositol trisphosphate with the subsequent activation of Ca2+-dependent K+ channels. Our data provide the first evidence that microglia express functional BK receptors and support the idea that microglia play an important role in CNS inflammatory responses.
| Original language | English |
|---|---|
| Pages (from-to) | 1573-1581 |
| Number of pages | 9 |
| Journal | Life Sciences |
| Volume | 72 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - Feb 21 2003 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
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Expression and function of bradykinin receptors in microglia. / Noda, Mami; Kariura, Yukihiro; Amano, Taiju; Manago, Yoshimasa; Nishikawa, Kaori; Aoki, Shunsuke; Wada, Keiji.
In: Life Sciences, Vol. 72, No. 14, 21.02.2003, p. 1573-1581.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and function of bradykinin receptors in microglia
AU - Noda, Mami
AU - Kariura, Yukihiro
AU - Amano, Taiju
AU - Manago, Yoshimasa
AU - Nishikawa, Kaori
AU - Aoki, Shunsuke
AU - Wada, Keiji
PY - 2003/2/21
Y1 - 2003/2/21
N2 - Expression of bradykinin (BK) receptors and their cellular function were investigated in microglia. Microglial cells were isolated from mixed cultures of cerebrocortical cells from postnatal day 3 Wistar rats. Reverse transcription-PCR (RT-PCR) showed that rat primary microglia express mRNAs for the type 2 bradykinin (B2) receptor subtype but not the type 1 (B1) receptor subtype under our experimental condition. However, the expression of B1 receptor was greatly up-regulated after the treatment of microglia with BK for 24 hours. The expression of B2 receptor in microglia was further confirmed by immunocytochemistry. Membrane currents were measured using whole-cell recording under voltage-clamp conditions. In 14% of patched cells (12/85 cells), BK (100-200 nM) induced an outward current at the holding potential of -20 mV, with oscillations in 2 cases. The BK-induced outward current was transient and desensitized rapidly. TEA inhibited the BK-induced outward current in a dose-dependent manner. These results suggest that microglia express B2 receptors and presumably increase the intracellular Ca2+ concentration via inositol trisphosphate with the subsequent activation of Ca2+-dependent K+ channels. Our data provide the first evidence that microglia express functional BK receptors and support the idea that microglia play an important role in CNS inflammatory responses.
AB - Expression of bradykinin (BK) receptors and their cellular function were investigated in microglia. Microglial cells were isolated from mixed cultures of cerebrocortical cells from postnatal day 3 Wistar rats. Reverse transcription-PCR (RT-PCR) showed that rat primary microglia express mRNAs for the type 2 bradykinin (B2) receptor subtype but not the type 1 (B1) receptor subtype under our experimental condition. However, the expression of B1 receptor was greatly up-regulated after the treatment of microglia with BK for 24 hours. The expression of B2 receptor in microglia was further confirmed by immunocytochemistry. Membrane currents were measured using whole-cell recording under voltage-clamp conditions. In 14% of patched cells (12/85 cells), BK (100-200 nM) induced an outward current at the holding potential of -20 mV, with oscillations in 2 cases. The BK-induced outward current was transient and desensitized rapidly. TEA inhibited the BK-induced outward current in a dose-dependent manner. These results suggest that microglia express B2 receptors and presumably increase the intracellular Ca2+ concentration via inositol trisphosphate with the subsequent activation of Ca2+-dependent K+ channels. Our data provide the first evidence that microglia express functional BK receptors and support the idea that microglia play an important role in CNS inflammatory responses.
UR - http://www.scopus.com/inward/record.url?scp=0037458425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037458425&partnerID=8YFLogxK
U2 - 10.1016/S0024-3205(02)02449-9
DO - 10.1016/S0024-3205(02)02449-9
M3 - Article
C2 - 12551746
AN - SCOPUS:0037458425
VL - 72
SP - 1573
EP - 1581
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 14
ER -