Expression and function of dopamine in odontoblasts

Shoko Fujino, Sayuri Hamano, Atsushi Tomokiyo, Tomohiro Itoyama, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Ayako Washio, Aoi Nozu, Taiga Ono, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda

Research output: Contribution to journalArticle

Abstract

Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.

Original languageEnglish
JournalJournal of cellular physiology
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Odontoblasts
Dopamine
Tyrosine 3-Monooxygenase
Dentinogenesis
Autocrine Communication
Paracrine Communication
Dopamine D2 Receptors
Dopamine Receptors
Incisor
Tyrosine
Rats

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Expression and function of dopamine in odontoblasts. / Fujino, Shoko; Hamano, Sayuri; Tomokiyo, Atsushi; Itoyama, Tomohiro; Hasegawa, Daigaku; Sugii, Hideki; Yoshida, Shinichiro; Washio, Ayako; Nozu, Aoi; Ono, Taiga; Wada, Naohisa; Kitamura, Chiaki; Maeda, Hidefumi.

In: Journal of cellular physiology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Sugii, Hideki

AU - Yoshida, Shinichiro

AU - Washio, Ayako

AU - Nozu, Aoi

AU - Ono, Taiga

AU - Wada, Naohisa

AU - Kitamura, Chiaki

AU - Maeda, Hidefumi

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