Expression and induction of CYP3As in human fetal hepatocytes

Tamihide Matsunaga, Masataka Maruyama, Eri Harada, Yoshihiko Katsuyama, Nobuhiro Sugihara, Hirohiko Ise, Naoki Negishi, Uichi Ikeda, Shigeru Ohmori

Research output: Contribution to journalArticle

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Abstract

CYP3A4 and CYP3A7 mRNA expression levels were markedly up-regulated by dexamethasone (DEX), but not by rifampicin (RIF). CYP3A5 mRNA level was not increased significantly by DEX, RIF, or phenobarbital. Testosterone 6β-hydroxylase activity was induced to about 2-fold of control by DEX. However, concomitant treatment with RIF did not alter DEX-mediated induction of CYP3A mRNA expression and testosterone 6β-hydroxylase activity. DEX-mediated induction of CYP3A mRNA was suppressed in a dose-dependent manner by RU486, a glucocorticoid receptor (GR) antagonist. At 5μM RU486, DEX-mediated induction of CYP3A4, CYP3A5, and CYP3A7 mRNA expression was inhibited almost completely. These results suggest that, in human fetal hepatocytes, PXR is not involved in DEX-mediated induction of CYP3A4 and CYP3A7, and that the induction is mediated directly by GR.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume318
Issue number2
DOIs
Publication statusPublished - May 28 2004

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Cytochrome P-450 CYP3A
Dexamethasone
Hepatocytes
Rifampin
Messenger RNA
Glucocorticoid Receptors
Mixed Function Oxygenases
Testosterone
Phenobarbital

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsunaga, T., Maruyama, M., Harada, E., Katsuyama, Y., Sugihara, N., Ise, H., ... Ohmori, S. (2004). Expression and induction of CYP3As in human fetal hepatocytes. Biochemical and Biophysical Research Communications, 318(2), 428-434. https://doi.org/10.1016/j.bbrc.2004.04.041

Expression and induction of CYP3As in human fetal hepatocytes. / Matsunaga, Tamihide; Maruyama, Masataka; Harada, Eri; Katsuyama, Yoshihiko; Sugihara, Nobuhiro; Ise, Hirohiko; Negishi, Naoki; Ikeda, Uichi; Ohmori, Shigeru.

In: Biochemical and Biophysical Research Communications, Vol. 318, No. 2, 28.05.2004, p. 428-434.

Research output: Contribution to journalArticle

Matsunaga, T, Maruyama, M, Harada, E, Katsuyama, Y, Sugihara, N, Ise, H, Negishi, N, Ikeda, U & Ohmori, S 2004, 'Expression and induction of CYP3As in human fetal hepatocytes', Biochemical and Biophysical Research Communications, vol. 318, no. 2, pp. 428-434. https://doi.org/10.1016/j.bbrc.2004.04.041
Matsunaga, Tamihide ; Maruyama, Masataka ; Harada, Eri ; Katsuyama, Yoshihiko ; Sugihara, Nobuhiro ; Ise, Hirohiko ; Negishi, Naoki ; Ikeda, Uichi ; Ohmori, Shigeru. / Expression and induction of CYP3As in human fetal hepatocytes. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 318, No. 2. pp. 428-434.
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