Expression and localization of versican during postnatal development of rat temporomandibular joint disc

Naoko Toriya, Taishin Takuma, Toshiya Arakawa, Yoshihiro Abiko, Yasuyuki Sasano, Ichiro Takahashi, Yasunori Sakakura, Firoz Rahemtulla, Itaru Mizoguchi

Research output: Contribution to journalArticle

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Abstract

To analyze the growth-related changes in extracellular matrix components in temporomandibular joint (TMJ) discs, the expression and localization of the core protein of a large chondroitin sulphate proteoglycan, versican, in rat TMJ discs during postnatal development (2-32 weeks) were examined using Western blot analysis, real-time quantitative PCR and immunohistochemistry. Western blot analysis showed that rat TMJ discs predominantly expressed one isoform (V1) and the core protein sharply increased after birth, reached a peak at 8 weeks, and then gradually decreased up to 32 weeks. Real-time quantitative PCR with TaqMan probes indicated that mRNA expression of versican was highest at 2 weeks and gradually decreased with growth. An immunohistochemical study showed that staining for versican was weak and evenly distributed in TMJ discs at 2 weeks. Regional differences in staining for versican became prominent after 8 weeks; staining was intense in the anterior and posterior peripheral attachments, and weak in the central part of the discs. These results demonstrate that growth-related changes and regional differences exist in the expression of versican in the TMJ discs of growing rats, and these probably reflect the changes in the biomechanical environment caused by the development of orofacial functions.

Original languageEnglish
Pages (from-to)205-214
Number of pages10
JournalHistochemistry and Cell Biology
Volume125
Issue number3
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

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Temporomandibular Joint Disc
Versicans
Staining and Labeling
Real-Time Polymerase Chain Reaction
Growth
Western Blotting
Chondroitin Sulfate Proteoglycans
Extracellular Matrix
Protein Isoforms
Proteins
Immunohistochemistry
Parturition
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Expression and localization of versican during postnatal development of rat temporomandibular joint disc. / Toriya, Naoko; Takuma, Taishin; Arakawa, Toshiya; Abiko, Yoshihiro; Sasano, Yasuyuki; Takahashi, Ichiro; Sakakura, Yasunori; Rahemtulla, Firoz; Mizoguchi, Itaru.

In: Histochemistry and Cell Biology, Vol. 125, No. 3, 01.03.2006, p. 205-214.

Research output: Contribution to journalArticle

Toriya, N, Takuma, T, Arakawa, T, Abiko, Y, Sasano, Y, Takahashi, I, Sakakura, Y, Rahemtulla, F & Mizoguchi, I 2006, 'Expression and localization of versican during postnatal development of rat temporomandibular joint disc', Histochemistry and Cell Biology, vol. 125, no. 3, pp. 205-214. https://doi.org/10.1007/s00418-005-0020-1
Toriya, Naoko ; Takuma, Taishin ; Arakawa, Toshiya ; Abiko, Yoshihiro ; Sasano, Yasuyuki ; Takahashi, Ichiro ; Sakakura, Yasunori ; Rahemtulla, Firoz ; Mizoguchi, Itaru. / Expression and localization of versican during postnatal development of rat temporomandibular joint disc. In: Histochemistry and Cell Biology. 2006 ; Vol. 125, No. 3. pp. 205-214.
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