Expression and purification of uniformly 15N-labeled amyloid β peptide 1-40 in Escherichia coli

M. Nagata-Uchiyama, M. Yaguchi, Y. Hirano, Tadashi Ueda

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining 15N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozyme as a fusion protein.

Original languageEnglish
Pages (from-to)788-792
Number of pages5
JournalProtein and Peptide Letters
Issue number8
Publication statusPublished - Aug 1 2007

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

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