Expression and Secretion of Bovine β-Lactoglobulin In Saccharomyces cerevisiae

Mamoru Totsuka, Yoshinori Katakura, Makoto Shimizu, Shuichi Kanminogawa, Izumi Kumagai, Kin ichiro Miura

Research output: Contribution to journalArticle

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Abstract

A bovine β-Mactoglobulin (β-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-β-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-β-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYG100, a yeast expression vector. In the supernatant of the yeast growth medium, β-LG with a native conformation was detected by sandwich ELISA, and its amount was estimated to be 1.1 mg/1. A Western-immunoblotting analysis revealed that β-LG secrected in the growth medium had the same mobility as that of authentic bovine β-LG. The N-terminal sequence was also identical with that of authentic mature bovine β-LG.

Original languageEnglish
Pages (from-to)3111-3116
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number12
DOIs
Publication statusPublished - Jan 1 1990

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lactoglobulins
Lactoglobulins
Yeast
Saccharomyces cerevisiae
secretion
culture media
Yeasts
cattle
yeasts
Growth
Complementary DNA
Glyceraldehyde-3-Phosphate Dehydrogenases
glyceraldehyde-3-phosphate dehydrogenase
sandwiches
immunoblotting
Conformations
plasmids
Plasmids
Genes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Expression and Secretion of Bovine β-Lactoglobulin In Saccharomyces cerevisiae. / Totsuka, Mamoru; Katakura, Yoshinori; Shimizu, Makoto; Kanminogawa, Shuichi; Kumagai, Izumi; Miura, Kin ichiro.

In: Agricultural and Biological Chemistry, Vol. 54, No. 12, 01.01.1990, p. 3111-3116.

Research output: Contribution to journalArticle

Totsuka, Mamoru ; Katakura, Yoshinori ; Shimizu, Makoto ; Kanminogawa, Shuichi ; Kumagai, Izumi ; Miura, Kin ichiro. / Expression and Secretion of Bovine β-Lactoglobulin In Saccharomyces cerevisiae. In: Agricultural and Biological Chemistry. 1990 ; Vol. 54, No. 12. pp. 3111-3116.
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