Expression levels of adiponectin receptors and periodontitis

N. Yamaguchi, T. Hamachi, N. Kamio, S. Akifusa, Keiji Masuda, Y. Nakamura, Kazuaki Nonaka, K. Maeda, S. Hanazawa, Yoshihisa Yamashita

Research output: Contribution to journalArticle

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Abstract

Background and Objective: We recently showed that adiponectin, an adipocyte-derived cytokine, may function as a negative regulator of the Toll-like receptor signaling pathway and of osteoclast formation in periodontal disease. In this study, we investigated whether the expression levels of adiponectin receptors (AdipoR1 and AdipoR2) are related to the presence of periodontitis. Material and Methods: We initially examined, using RT-PCR, the expression of the AdipoR1 and AdipoR2 genes at the mRNA level in several oral tissues of C57BL mice. Next, we investigated (using real-time PCR assays) whether inflammatory cytokines, such as tumor necrosis factor-α, could affect the expression levels of these genes in human gingival fibroblasts. Lastly, we compared the expression levels of these receptor proteins in gingival tissues between two healthy subjects and five patients with severe periodontal disease using western blotting analysis. Results: The AdipoR1 and AdipoR2 receptors were ubiquitously expressed in the oral tissues of mice. We observed that treatment with tumor necrosis factor-α could significantly reduce the expression levels of both AdipoR1 and AdipoR2 genes in human gingival fibroblasts. Moreover, we found that the expression of both receptors was lower in periodontal tissues from patients with severe periodontitis than in patients with healthy periodontal tissues. Conclusion: These observations suggest that adiponectin may not function efficiently in sites of periodontal disease because of a decrease in the number of its receptors, and this probable dysfunction may play a role in worsening periodontitis in patients.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalJournal of Periodontal Research
Volume45
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

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Adiponectin Receptors
Periodontitis
Periodontal Diseases
Adiponectin
Tumor Necrosis Factor-alpha
Fibroblasts
Cytokines
Toll-Like Receptors
Osteoclasts
Inbred C57BL Mouse
Adipocytes
Genes
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Western Blotting
Gene Expression
Polymerase Chain Reaction
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Periodontics

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Expression levels of adiponectin receptors and periodontitis. / Yamaguchi, N.; Hamachi, T.; Kamio, N.; Akifusa, S.; Masuda, Keiji; Nakamura, Y.; Nonaka, Kazuaki; Maeda, K.; Hanazawa, S.; Yamashita, Yoshihisa.

In: Journal of Periodontal Research, Vol. 45, No. 2, 01.04.2010, p. 296-300.

Research output: Contribution to journalArticle

Yamaguchi, N, Hamachi, T, Kamio, N, Akifusa, S, Masuda, K, Nakamura, Y, Nonaka, K, Maeda, K, Hanazawa, S & Yamashita, Y 2010, 'Expression levels of adiponectin receptors and periodontitis', Journal of Periodontal Research, vol. 45, no. 2, pp. 296-300. https://doi.org/10.1111/j.1600-0765.2009.01222.x
Yamaguchi, N. ; Hamachi, T. ; Kamio, N. ; Akifusa, S. ; Masuda, Keiji ; Nakamura, Y. ; Nonaka, Kazuaki ; Maeda, K. ; Hanazawa, S. ; Yamashita, Yoshihisa. / Expression levels of adiponectin receptors and periodontitis. In: Journal of Periodontal Research. 2010 ; Vol. 45, No. 2. pp. 296-300.
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