Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Yasuhiro Horibata, Noriyuki Sueyoshi, Makoto Ito

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number2
DOIs
Publication statusPublished - Mar 1 2007

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Sphingomyelin Phosphodiesterase
Pseudomonas
Cells
Glucosylceramides
Sphingomyelins
Ceramides
Acid Phosphatase
Lysosomes
Sorting

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells. / Horibata, Yasuhiro; Sueyoshi, Noriyuki; Ito, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 71, No. 2, 01.03.2007, p. 603-606.

Research output: Contribution to journalArticle

Horibata, Yasuhiro ; Sueyoshi, Noriyuki ; Ito, Makoto. / Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells. In: Bioscience, Biotechnology and Biochemistry. 2007 ; Vol. 71, No. 2. pp. 603-606.
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