Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Yasuhiro Horibata; Noriyuki Sueyoshi; Makoto Ito

doi:10.1271/bbb.60544

Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Yasuhiro Horibata, Noriyuki Sueyoshi, Makoto Ito

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

Original language	English
Pages (from-to)	603-606
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	71
Issue number	2
DOIs	https://doi.org/10.1271/bbb.60544
Publication status	Published - 2007

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells",

abstract = "We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.",

author = "Yasuhiro Horibata and Noriyuki Sueyoshi and Makoto Ito",

note = "Funding Information: We are grateful to Dr. Yoshitaka Tanaka (Kyushu University) for donating LAP. This work was supported in part by Grants-in-aid for Basic Research B 17380068 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.",

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T1 - Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

AU - Horibata, Yasuhiro

AU - Sueyoshi, Noriyuki

AU - Ito, Makoto

N1 - Funding Information: We are grateful to Dr. Yoshitaka Tanaka (Kyushu University) for donating LAP. This work was supported in part by Grants-in-aid for Basic Research B 17380068 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.

PY - 2007

Y1 - 2007

N2 - We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

AB - We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

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ER -

Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Abstract

All Science Journal Classification (ASJC) codes

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