Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Yasuhiro Horibata, Noriyuki Sueyoshi, Makoto Ito

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number2
DOIs
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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