Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Minoru Ujita, Mayumi Yamanaka, Yumiko Maeno, Koki Yoshida, Wakana Ohshio, Yoshinori Ueno, Yutaka Banno, Hiroshi Fujii, Hiroki Okumura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.

Original languageEnglish
Pages (from-to)22-25
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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Trehalase
Bombyx
Baculoviridae
Polysaccharides
His-His-His-His-His-His
Fusion reactions
Proteins
Insects

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. / Ujita, Minoru; Yamanaka, Mayumi; Maeno, Yumiko; Yoshida, Koki; Ohshio, Wakana; Ueno, Yoshinori; Banno, Yutaka; Fujii, Hiroshi; Okumura, Hiroki.

In: Journal of Bioscience and Bioengineering, Vol. 111, No. 1, 01.01.2011, p. 22-25.

Research output: Contribution to journalArticle

Ujita, Minoru ; Yamanaka, Mayumi ; Maeno, Yumiko ; Yoshida, Koki ; Ohshio, Wakana ; Ueno, Yoshinori ; Banno, Yutaka ; Fujii, Hiroshi ; Okumura, Hiroki. / Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. In: Journal of Bioscience and Bioengineering. 2011 ; Vol. 111, No. 1. pp. 22-25.
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