Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Minoru Ujita, Mayumi Yamanaka, Yumiko Maeno, Koki Yoshida, Wakana Ohshio, Yoshinori Ueno, Yutaka Banno, Hiroshi Fujii, Hiroki Okumura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.

Original languageEnglish
Pages (from-to)22-25
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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