Expression of adipose differentiation-related protein (ADRP) and perilipin in macrophages infected with Mycobacterium leprae

Kazunari Tanigawa, Koichi Suzuki, Kazuaki Nakamura, Takeshi Akama, Akira Kawashima, Huhehasi Wu, Moyuru Hayashi, Shin Ichiro Takahashi, Shoichiro Ikuyama, Tetsuhide Ito, Norihisa Ishii

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Mycobacterium leprae survives and replicates within a lipid droplet stored in the enlarged phagosome of histiocytes, a typical feature of lepromatous leprosy that is thought to be an important nutrient source for the bacillus. However, the underlying mechanisms by which lipids accumulate within phagosomes remain unclear. Recently, it was revealed that the lipid droplet-associated proteins, including ADRP and perilipin, play essential roles in lipid accumulation in adipocytes or macrophages. Therefore, we attempted to examine the role of these proteins in leprosy pathogenesis. ADRP and perilipin localized to the phagosomal membrane, which contains M. leprae in skin biopsy specimens of lepromatous leprosy. ADRP expression was transiently increased after phagocytosis in THP-1 cells. However, high levels of ADRP expression persisted only when live M. leprae, but not dead bacilli or latex beads, was added. Furthermore, although peptidoglycan, a Toll-like receptor 2 ligand, suppressed the expression levels of ADRP and perilipin, M. leprae infection inhibited this suppression. These results suggest that live M. leprae has the ability to actively induce and support ADRP/perilipin expression to facilitate the accumulation of lipids within the phagosome and to further maintain a suitable environment for the intracellular survival within the macrophage.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalFEMS microbiology letters
Volume289
Issue number1
DOIs
Publication statusPublished - Dec 1 2008

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Mycobacterium leprae
Macrophages
Phagosomes
Lepromatous Leprosy
Lipids
Bacillus
Toll-Like Receptor 2
Mycobacterium Infections
Histiocytes
Peptidoglycan
Leprosy
Microspheres
Phagocytosis
Adipocytes
Perilipin-1
Perilipin-2
Ligands
Biopsy
Food
Skin

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Expression of adipose differentiation-related protein (ADRP) and perilipin in macrophages infected with Mycobacterium leprae. / Tanigawa, Kazunari; Suzuki, Koichi; Nakamura, Kazuaki; Akama, Takeshi; Kawashima, Akira; Wu, Huhehasi; Hayashi, Moyuru; Takahashi, Shin Ichiro; Ikuyama, Shoichiro; Ito, Tetsuhide; Ishii, Norihisa.

In: FEMS microbiology letters, Vol. 289, No. 1, 01.12.2008, p. 72-79.

Research output: Contribution to journalArticle

Tanigawa, K, Suzuki, K, Nakamura, K, Akama, T, Kawashima, A, Wu, H, Hayashi, M, Takahashi, SI, Ikuyama, S, Ito, T & Ishii, N 2008, 'Expression of adipose differentiation-related protein (ADRP) and perilipin in macrophages infected with Mycobacterium leprae', FEMS microbiology letters, vol. 289, no. 1, pp. 72-79. https://doi.org/10.1111/j.1574-6968.2008.01369.x
Tanigawa, Kazunari ; Suzuki, Koichi ; Nakamura, Kazuaki ; Akama, Takeshi ; Kawashima, Akira ; Wu, Huhehasi ; Hayashi, Moyuru ; Takahashi, Shin Ichiro ; Ikuyama, Shoichiro ; Ito, Tetsuhide ; Ishii, Norihisa. / Expression of adipose differentiation-related protein (ADRP) and perilipin in macrophages infected with Mycobacterium leprae. In: FEMS microbiology letters. 2008 ; Vol. 289, No. 1. pp. 72-79.
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