TY - JOUR
T1 - Expression of AML/Runx and ETO/MTG family members during hematopoietic differentiation of embryonic stem cells
AU - Okumura, Akiko Joo
AU - Peterson, Luke F.
AU - Lo, Miao Chia
AU - Zhang, Dong Er
N1 - Funding Information:
We thank Dr. A. Boyapati for helpful suggestions with the real-time PCR technique and critical reading of this manuscript and Dr. Joseph Biggs for editing this manuscript. AJO was supported by the Japan Society for Promotion of Science and the Mochida Memorial Foundation. This work was supported by National Institutes of Health grant CA096735 (DEZ). MCL is a fellow of the Leukemia and Lymphoma Society of North America. The Stein Endowment Fund has partially supported the departmental molecular biology service laboratory for DNA sequencing and oligonucleotide synthesis. This is manuscript 18140-MEM from The Scripps Research Institute.
PY - 2007/6
Y1 - 2007/6
N2 - Runx1/AML1 plays important roles in hematopoiesis, including the commitment of cells to hematopoiesis during embryonic development, and in the maintenance of hematopoietic cell populations. It is also one of the most common genes involved in chromosomal translocations related to leukemia. One such translocation is t(8;21), which fuses the Runx1 gene to the MTG8/ETO gene and generates the Runx1-MTG8 (AML1-ETO) fusion gene. Both Runx1 and MTG8 have two additional family members that are much less studied in hematopoiesis. Here we report the expression of every member of the Runx and MTG families as well as the Runx heterodimerization partner CBFβ during hematopoietic differentiation of murine embryonic stem cells. We observed substantially increased expression of Runx1, Runx2, and MTG16 during hematopoietic differentiation. Furthermore, the increase in Runx2 expression is delayed relative to Runx1 expression, suggesting their possible sequential contribution to hematopoiesis.
AB - Runx1/AML1 plays important roles in hematopoiesis, including the commitment of cells to hematopoiesis during embryonic development, and in the maintenance of hematopoietic cell populations. It is also one of the most common genes involved in chromosomal translocations related to leukemia. One such translocation is t(8;21), which fuses the Runx1 gene to the MTG8/ETO gene and generates the Runx1-MTG8 (AML1-ETO) fusion gene. Both Runx1 and MTG8 have two additional family members that are much less studied in hematopoiesis. Here we report the expression of every member of the Runx and MTG families as well as the Runx heterodimerization partner CBFβ during hematopoietic differentiation of murine embryonic stem cells. We observed substantially increased expression of Runx1, Runx2, and MTG16 during hematopoietic differentiation. Furthermore, the increase in Runx2 expression is delayed relative to Runx1 expression, suggesting their possible sequential contribution to hematopoiesis.
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U2 - 10.1016/j.exphem.2007.03.002
DO - 10.1016/j.exphem.2007.03.002
M3 - Article
C2 - 17533052
AN - SCOPUS:34249110657
VL - 35
SP - 978
EP - 988
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 6
ER -
