Expression of an X-family DNA polymerase, pol lambda, in the respiratory epithelium of non-small cell lung cancer patients with habitual smoking

Taro Ohba, Takurou Kometani, Fumihiro Shoji, Tokujiro Yano, Yoshino Ichiro, Kenichi Taguchi, Isao Kuraoka, Shinya Oda, Yoshihiko Maehara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

DNA polymerase lambda, pol λ, is a eukaryotic member of the X-family DNA polymerases that is involved in two modes of DNA repair, i.e. base excision repair (BER) or non-homologous end joining (NHEJ). Using immunohistochemical approaches, we have observed pol λ expression in human tissues, particularly in the respiratory system of lung cancer patients. pol λ proteins were distributed in the nuclei of the epithelial cells in the bronchi, bronchioles and alveoli. Intriguingly, the level of pol λ expression in the bronchiolar epithelia significantly correlated with the amount of habitual smoking in the individuals. Conversely, pol λ expression in cancer tissues did not correlate with the smoking status of the patients. Pol λ expression was sometimes discrepant between the tumor tissues and adjacent bronchioles. More importantly, tumors without pol λ expression that occurred in heavy smokers significantly tended to be at an advanced clinical stage. Pol λ may thus be involved in the DNA repair processes counteracting DNA damage caused by tobacco smoke in the respiratory system.

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume677
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2009

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Respiratory Mucosa
Non-Small Cell Lung Carcinoma
DNA Repair
Bronchioles
Smoking
Respiratory System
pol Gene Products
Neoplasms
Bronchi
Smoke
DNA Damage
Tobacco
Lung Neoplasms
Epithelium
Epithelial Cells
DNA polymerase X
DNA polymerase beta2

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

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Expression of an X-family DNA polymerase, pol lambda, in the respiratory epithelium of non-small cell lung cancer patients with habitual smoking. / Ohba, Taro; Kometani, Takurou; Shoji, Fumihiro; Yano, Tokujiro; Ichiro, Yoshino; Taguchi, Kenichi; Kuraoka, Isao; Oda, Shinya; Maehara, Yoshihiko.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 677, No. 1-2, 01.01.2009, p. 66-71.

Research output: Contribution to journalArticle

Ohba, Taro ; Kometani, Takurou ; Shoji, Fumihiro ; Yano, Tokujiro ; Ichiro, Yoshino ; Taguchi, Kenichi ; Kuraoka, Isao ; Oda, Shinya ; Maehara, Yoshihiko. / Expression of an X-family DNA polymerase, pol lambda, in the respiratory epithelium of non-small cell lung cancer patients with habitual smoking. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2009 ; Vol. 677, No. 1-2. pp. 66-71.
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AU - Ichiro, Yoshino

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AU - Kuraoka, Isao

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