Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA

Miyuki Tsuda, Koichiro Matsumoto, Hiromasa Inoue, Mikiko Matsumura, Takako Nakano, Akio Mori, Miyuki Azuma, Yoichi Nakanishi

Research output: Contribution to journalArticle

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Abstract

Viral infection in the airway provokes various immune responses, including Th1 and Th2 responses, which are partly initiated by double-stranded RNA (dsRNA), a viral product for its replication. B7-H1 (PD-L1) and B7-DC (PD-L2) are B7-family molecules that bind to programmed death-1 (PD-1) on lymphocytes and are implicated in peripheral tolerance. We investigated the effect of dsRNA on the expression of B7-H1 and B7-DC on airway epithelial cell lines. B7-H1 and B7-DC were constitutively expressed on the cells, and their expression was profoundly upregulated by stimulation with an analog of viral dsRNA, polyinosinic-polycytidylic acid. B7-H1 and B7-DC were also upregulated by stimulation with IFN-γ, IL-13, and the supernatant from T cell clones. A relatively high concentration of dexamethasone (1 μM) was required to suppress the upregulation of B7-H1 or B7-DC. These results suggest that epithelial B7-H1 and B7-DC play a role in virus-associated immune responses in the airways.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number1
DOIs
Publication statusPublished - Apr 29 2005

Fingerprint

Double-Stranded RNA
Epithelium
B7 Antigens
Peripheral Tolerance
Poly I-C
T-cells
Interleukin-13
Lymphocytes
Viral RNA
Virus Diseases
Viruses
Dexamethasone
Up-Regulation
Clone Cells
Epithelial Cells
T-Lymphocytes
Cell Line
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA. / Tsuda, Miyuki; Matsumoto, Koichiro; Inoue, Hiromasa; Matsumura, Mikiko; Nakano, Takako; Mori, Akio; Azuma, Miyuki; Nakanishi, Yoichi.

In: Biochemical and Biophysical Research Communications, Vol. 330, No. 1, 29.04.2005, p. 263-270.

Research output: Contribution to journalArticle

Tsuda, Miyuki ; Matsumoto, Koichiro ; Inoue, Hiromasa ; Matsumura, Mikiko ; Nakano, Takako ; Mori, Akio ; Azuma, Miyuki ; Nakanishi, Yoichi. / Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 330, No. 1. pp. 263-270.
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