Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma

Jian Li, Yi Yang, Tatsuro Fujie, Kinya Baba, Hiroaki Ueo, Masaki Mori, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticle

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Abstract

The MAGE, BAGE, and GAGE genes code for distinct antigens that are recognized by autologous cytolytic T lymphocytes. We investigated the expression of these genes in both cell lines and surgical samples of gastric carcinoma, using reverse transcription-PCR. Furthermore, the induction of these genes by 5-aza-2′-deoxycytidine (DAC), a demethylating agent, was also examined in several cell lines. Of 11 cell lines, BAGE, GAGE1-6, GAGE1-2, MAGE-1, and MAGE-3 were detected in 7 (64%), 4 (36%), 3 (27%), 8 (73%), and 8 (73%) cell lines, respectively. After the in vitro treatment of the negative cell lines with DAC, the expression of these genes became positive in 46 to 91% of these cell lines. No expression of these genes was seen in any of the 57 samples of normal gastric tissue. In contrast, the tumor tissue samples expressed BAGE, GAGE1-6, GAGE1-2, MAGE-1, and MAGE-3 in 13 (23%), 9 (16%), 6 (11%), 25 (44%), and 23 (40%) tissue samples, respectively. Thus, at least one of these genes was expressed in 35 (61%) of 57 carcinomas. An analysis of the relationship between clinico-pathological factors and the expression of these genes revealed that either BAGE or one of these genes was more frequently expressed in histologically intestinal-type than in diffuse-type carcinomas. Our results suggest that, because of the higher expression of these genes and the possible induction of these genes by DAC, patients with gastric carcinoma may, therefore, be potential candidates for tumor-specific immunotherapy directed against these antigens.

Original languageEnglish
Pages (from-to)1619-1625
Number of pages7
JournalClinical Cancer Research
Volume2
Issue number9
Publication statusPublished - Sep 1 1996

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Stomach
Carcinoma
Cell Line
Gene Expression
Genes
decitabine
Autoantigens
Immunotherapy
Reverse Transcription
Neoplasms
T-Lymphocytes
Antigens
Polymerase Chain Reaction
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Li, J., Yang, Y., Fujie, T., Baba, K., Ueo, H., Mori, M., & Akiyoshi, T. (1996). Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma. Clinical Cancer Research, 2(9), 1619-1625.

Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma. / Li, Jian; Yang, Yi; Fujie, Tatsuro; Baba, Kinya; Ueo, Hiroaki; Mori, Masaki; Akiyoshi, Tsuyoshi.

In: Clinical Cancer Research, Vol. 2, No. 9, 01.09.1996, p. 1619-1625.

Research output: Contribution to journalArticle

Li, J, Yang, Y, Fujie, T, Baba, K, Ueo, H, Mori, M & Akiyoshi, T 1996, 'Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma', Clinical Cancer Research, vol. 2, no. 9, pp. 1619-1625.
Li J, Yang Y, Fujie T, Baba K, Ueo H, Mori M et al. Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma. Clinical Cancer Research. 1996 Sep 1;2(9):1619-1625.
Li, Jian ; Yang, Yi ; Fujie, Tatsuro ; Baba, Kinya ; Ueo, Hiroaki ; Mori, Masaki ; Akiyoshi, Tsuyoshi. / Expression of BAGE, GAGE, and MAGE Genes in Human Gastric Carcinoma. In: Clinical Cancer Research. 1996 ; Vol. 2, No. 9. pp. 1619-1625.
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abstract = "The MAGE, BAGE, and GAGE genes code for distinct antigens that are recognized by autologous cytolytic T lymphocytes. We investigated the expression of these genes in both cell lines and surgical samples of gastric carcinoma, using reverse transcription-PCR. Furthermore, the induction of these genes by 5-aza-2′-deoxycytidine (DAC), a demethylating agent, was also examined in several cell lines. Of 11 cell lines, BAGE, GAGE1-6, GAGE1-2, MAGE-1, and MAGE-3 were detected in 7 (64{\%}), 4 (36{\%}), 3 (27{\%}), 8 (73{\%}), and 8 (73{\%}) cell lines, respectively. After the in vitro treatment of the negative cell lines with DAC, the expression of these genes became positive in 46 to 91{\%} of these cell lines. No expression of these genes was seen in any of the 57 samples of normal gastric tissue. In contrast, the tumor tissue samples expressed BAGE, GAGE1-6, GAGE1-2, MAGE-1, and MAGE-3 in 13 (23{\%}), 9 (16{\%}), 6 (11{\%}), 25 (44{\%}), and 23 (40{\%}) tissue samples, respectively. Thus, at least one of these genes was expressed in 35 (61{\%}) of 57 carcinomas. An analysis of the relationship between clinico-pathological factors and the expression of these genes revealed that either BAGE or one of these genes was more frequently expressed in histologically intestinal-type than in diffuse-type carcinomas. Our results suggest that, because of the higher expression of these genes and the possible induction of these genes by DAC, patients with gastric carcinoma may, therefore, be potential candidates for tumor-specific immunotherapy directed against these antigens.",
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